摘要
目的研究覆盆子酮对糖尿病小鼠的降血糖作用及其机制。方法以四氧嘧啶尾静脉注射诱导糖尿病小鼠模型,随机分为模型组、阳性对照组(二甲双胍,90 mg.kg-1)、覆盆子酮低、中、高剂量组(200,400,800 mg.kg-1),同时设正常对照组,灌胃给药,连续给药20 d。在实验第0、7、14天各测空腹血糖;实验末期进行:糖耐量实验;测定血清胰岛素水平、计算胰岛素敏感指数(ISI);测定血清超氧化物歧化酶、谷胱甘肽过氧化物酶活性和丙二醛水平;通过胰腺HE染色和胰岛素免疫组化分析观察胰腺组织病变程度和胰岛β细胞胰岛素表达。结果覆盆子酮具有明显控制糖尿病小鼠空腹血糖值(P<0.05)升高,降低血糖曲线下面积的作用,其中覆盆子酮高剂量组降糖作用与阳性对照组相当;覆盆子酮高剂量组显著提高血清胰岛素水平(P<0.05);显著增加血清中超氧化物歧化酶和谷胱甘肽的含量(P<0.05),并能显著降低血清中丙二醛的含量(P<0.05);HE及免疫组化分析表明,覆盆子酮有效修复胰岛病理改变,增加胰岛β细胞中胰岛素的表达。结论覆盆子酮具有剂量依赖性地降血糖效果,增加胰岛素分泌,各剂量对四氧嘧啶造成的胰腺损伤都有一定修复作用。机制可能与提高糖尿病小鼠抗氧化能力,促进胰岛β细胞修复有关。
OBJECTIVE To observe the hypoglycemic effect of raspberry ketone and its mechanism on diabetic model mice in- duced by alloxan. METHODS Healthy male KM mice were used to establish diabetic models by injecting alloxan via tail vein, and then randomly divided into model control group, metformin group （90 mg·kg^-1 ） , and raspberry ketone low, middle and high （200, 400, 800 mg ·kg^-1） dose groups. Normal control group was set. All groups had been treated for 20 d. Fasting blood glucose （FBG） was measured on 0, 7 and 14 d. After 20 d, glucose tolerance test was carried out. Fast insulin （FINS） of the mice were measured, and the insulin sensitivity index（ISI） was calculated by determining the contents of FBG and FINS. Superoxide dismutase （SOD）, glu- tathione peroxidase （GSH-Px）, and malonaldehyde （MDA） in serum were measured. Pathological changes of pancreas and insulin ex- pression were examined. RESULTS Raspberry ketone could effectively control the increase of fasting plasma glucose （P 〈 0. 05 ） and reduce the role of the area under the glucose curve. Compared with the model group, the levels of FINS, SOD and GSH-Px activity were significantly improved （P 〈 0. 05）, and MDA content was decreased （P 〈 0. 05） in the high dose raspberry ketone group. The pathologi- cal symptoms of pancreas were relieved in the high dose group. CONCLUSION Raspberry ketone can effectively control blood glucose, orotect oancreatic islet B ceils, and imorove insulin secretion in diabetic mice bv effectivelv inhibiting the oxidative stress.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2012年第23期1899-1904,共6页
Chinese Pharmaceutical Journal
基金
农业部都市农业重点开发实验室开发研究项目(NYBBF-2011-R001)
关键词
覆盆子酮
糖尿病
氧化应激
胰岛病理
raspberry ketone
diabetes mellitus
oxidative stress
islet pathology
作者简介
谢欣梅，女，硕士，讲师研究方向：药物评价
通讯作者：庞晓斌，男，博士，副教授研究方向：药物筛选、生物制药Tel：（0378）3880680E—mail：hndxpxb@163．com
