摘要
目的建立快速、准确分析山银花水提取液的化学成分的高效液相色谱串联质谱方法。方法采用水为溶剂加热回流提取法提取山银花药材,提取液采用高效液相色谱串联质谱法对其主要的化学成分进行分离和鉴定。结果在负离子模式下,21个主要的化学成分得到了较好的分离,其中16个通过串联质谱进行了结构鉴定,10个通过对照品得到了确证。结论高效液相色谱串联质谱法可有效用于山银花药材的化学成分的分析鉴定。
Objective To establish a high-performance liquid chromatogranphy coupled with tandem mass spectrometry（HPLC-MSn） method for analyzing the chemical constituents from the water extract of Lonicerae Flos rapidly and accurately.Methods Lonicerae Flos was extracted by reflux extraction using water as solvent,and the extract was separated and identified by HPLC-MSn.Results Under the negative-ion mode,21 major constituents were well separated,16 of them were identified by tandem mass spectrometer and 10 of them were confirmed by a comparison.Conclusion The HPLC-MSn method is useful for the rapid identification of the chemical constituents from Lonicerae Flos.
出处
《中国医药导报》
CAS
2012年第24期113-114,117,共3页
China Medical Herald
基金
2011年国家工程研究中心创新能力建设项目
关键词
山银花
水提取液
化学成分
高效液相色谱串联质谱法
Lonicerae Flos
Water extract
Components
High-performance liquid chromatography coupled with tandem mass spectrometry
作者简介
张湘东（1971．5-），男，硕士研究生，制药工程师，主要从事中药／天然药物的研究与开发工作。
[通讯作者]钟鸣（1973．2-），男，硕士研究生，制药高级工程师，主要从事中药／天然药物的研究与开发工作。
