摘要
目的:通过固态发酵筛选能使附子减毒增效的微生物,并初步探讨其生物转化机制。方法:选用附子粉为原料,以菌种生长情况、双酯型生物碱含量变化及物质成分变化为评价指标,通过固态发酵方式筛选适合附子生物转化的菌种。采用HPLC-MS初步鉴定新增物质的结构,为附子生物转化的可行性提供理论依据。结果:采用黑曲霉对附子进行生物转化,可使乌头碱含量减少,新增1种毒性较小的苯甲酰乌头原碱。结论:黑曲霉发酵附子实现了减毒增效的目的,为附子炮制新方法的深入研究提供理论依据。
Objective： To screen microbial with attenuated efficiency for Aconitum carmichaeli by solid- state fermentation, and preliminary explore its biotransformation mechanism. powder as raw material, bacteria growth, the content of diester alkaloid and Method： With A. carmichaeli material composition changes as evaluation indexes, suitable bacteria for biotransformation of A. carmichaeli was screened by solid-state fermentation. Structure of new substances were preliminary identified by HPLC.-MS, and in order to provide a theoretical basis for biotransformation feasibility of A. carmichaeli. Result： Biotransformation with aspergillus could reduce the content of aconitine, and added a new compound of benzoylaconitine with less toxic. Conclusion： Benzoylaconitine could achieve purpose of attenuated efficiency for A. carmichaeli, which provided a theoretical basis and feasibility to depth study of new processing method for A. carmichaeli.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第22期16-19,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
教育部春晖计划项目(Z2010102)
四川省中医药管理局项目(2010-69)
关键词
生物转化
附子
黑曲霉
减毒增效
biotransformation
Aconitum carmichaeli
aspergillus
attenuated efficiency
作者简介
孙鹏，硕士，Tel：15882189401，E-mail：793243748@qq．com
[通讯作者】李玲，博士，副教授，从事药物新制剂与新剂型研究，Tel：028-87720584，E-mail：lilyll88@126．com
