摘要
建立了薄层荧光扫描法测定中药喜树果中喜树碱和10-羟基喜树碱的方法。以无水甲醇为溶剂从喜树果样品中提取喜树碱和10-羟基喜树碱。在硅胶H板上以氯仿∶丙酮(7∶3)为展开剂可使喜树碱与10-羟基喜树碱很好地分离。以荧光激发波长350 nm线性扫描进行定量分析。在0.010 5～0.063 0μg范围内,喜树碱的积分荧光强度A与其质量m呈线性,相关系数为0.998,加标回收率为99%。在0.003 3～0.033 0μg范围内,10-羟基喜树碱的积分荧光强度与质量呈线性,相关系数为0.999,加标回收率为96%。该方法简便,重复性好。以此方法测得喜树果样品中喜树碱和10-羟基喜树碱的含量分别为0.122%和0.013%。
A thin layer chromatography（TLC） -fluorescence scanning method was established for the determination of camptothecine （CPT） and 10-hydroxycamptothecine＇（10-HCPT） in Chinese herbal medicine Xishuguo（ Common camptotheca fruit, CCF）. Methanol was used as solvent to extract CPT and 10-HCPT in CCF sample. The separation was carried out on silica gel H plate using chloroform - acetone（7 ： 3 ） as developing agent. The determination was accomplished by fluorescence linear scanning with excitation wavelength of 350 nm. In the quality range of 0. 010 5 -0. 063 0 μg, there is a linear relationship between integral fluorescence intensity and quality of CPT. The regression equation was A =474. 0 ＋ 159 881.2 m（μg） with a correlation coefficient of 0. 998. The spiked re- covery of CPT was 99%. In the quality range of 0. 003 3 - 0. 033 0 μg, a linear relationship between integral fluorescence intensity and quality of 10-HCPT was observed, and the regression equation was A = 51.3 ＋ 59 486. 2 m （μg） with a correlation coefficient 0. 999. The spiked recovery of 10-HCPT was 96%. This method was simple and repeatable. Using this method, the contents of CPT and 10-HCPT in a CCF sample were determined to be 0. 122% and 0. 013% , respectively.
出处
《分析测试学报》
CAS
CSCD
北大核心
2012年第10期1282-1287,共6页
Journal of Instrumental Analysis
基金
国家自然科学基金资助(20675025,20975029)
关键词
中药
喜树果
喜树碱
10-羟基喜树碱
薄层荧光扫描
traditional Chinese medicine
common camptotheca fruit
camptothecine
lO-hydroxy-camptothecine
TLC - fluorescence scanning
作者简介
通讯作者：魏永巨，博士，教授，研究方向：中药荧光分析：0311—87861162，E—mail：weiyju@126．com
