摘要
建立了喜树果中喜树碱的高效毛细管电泳安培检测方法。着重探讨了缓冲溶液种类、浓度、酸碱度及操作电压、进样时间等对检测的影响。Na2B4O7-NaOH（Na2B4O7浓度为30mmol／L）为电泳介质，在15kV高压，pH9.0的碱性条件下片柱端安培检测喜树碱含量。该法的线性范围为：1-100mg／L，Y=11020＋1014.6P，r=0.9994，检出限为0.5mg／L。
Camptothecin from Fructus Camptothecae Acuminatae was determined by capillary electro- phoresis with amperometric deteetion（CE/AD）. The buffer pH value, the types of buffer solution, the concentration of the buffer, the applied voltage and the sampling time were selected as the investi-gation variables. Under the optimized eonditions： a running buffer composed of 30 mmol/L Na2B4O7 at pH 9.0, separation voltage at 15 kV, injection time for 10 s and detection voltage at 0.8 V, a line-arity exists between peak area（Y） and mass eoncentration of camptotheein（p） in the range of 1-100mg/L, Y= 11020 ＋ 1014.6 p, r =0.9994. The deteetion limit is 0.5 mg/L.
出处
《分析测试学报》
CAS
CSCD
北大核心
2008年第3期319-321,共3页
Journal of Instrumental Analysis
关键词
毛细管电泳
喜树碱
伏安检测
capillary electrophoresis
camptothecin
amperometric detection
作者简介
黄宝美（1979-），女，山东沂水人，硕士，Tel：0816-8758582，E-mail：hbm790117@yahoo．com．cn
