摘要
利用致病谱较广的稻瘟病代表菌株GD0193于水稻品种泰航68空间诱变当代(SP1代)群体中筛选抗病突变体,并对其作初步的抗性遗传分析、抗病基因定位及苗期抗谱、田间穗颈瘟抗性分析。结果表明:空间诱变可对泰航68的稻瘟病抗性进行有效诱变,研究获得的抗病突变体TH1及TH2于SP2代对菌株GD0193分别表现抗性不分离及抗性分离,且TH2的抗性分离比例符合显性单基因抗性遗传规律,并已将该抗病基因初步定位于第11染色体长臂上;TH1及TH2的抗性可稳定遗传至后代,其苗期抗谱及田间穗颈瘟抗性均较原种对照表现显著提高,且其抗性较华南稻区的部分主栽品种具不同程度的提高。
To screen the blast resistance mutants,the resistance of SP1 progenies derived from rice variety Taihang-68 were evaluated after satellite flight by representative blast isolate GD0193 which had a broad pathogenic spectra,and then primary genetic analysis of resistant mutants and mapping of resistance gene,as well as resistance spectra at seedling and neck blast resistance at maturity were performed.The results showed that space-mutation was effective method to change the blast resistance of Taihang-68.The screened resistant mutants TH1and TH2 showed the resistance to isolate GD0193 no disjunction and separation respectively,and the resistance separation ratio of TH2 indicated that its resistance was controlled by one pair of major genes,which was preliminary mapped on the long arm of chromosome 11.In blast resistance spectra and neck blast resistance,TH1 and TH2 were both enhanced remarkably compared with the wild-type at seedling and maturity,and their resistance could be inherited,the blast resistance of these two mutants were also increased comparing with several main cultivars in South China.
出处
《核农学报》
CAS
CSCD
北大核心
2012年第5期734-739,共6页
Journal of Nuclear Agricultural Sciences
基金
国家863高新技术发展计划项目(2007AA100101)
“十一五”国家科技支撑计划重点项目(2008BAD97B02)
广东省自然科学基金项目(S2011020001513)
国家和广东省现代农业产业技术体系建设专项资金
关键词
空间诱变
泰航68
稻瘟病
抗性突变
抗性遗传分析
space-mutation
Taihang-68
rice blast
resistance variation
resistance genetic analysis
作者简介
张景欣(1984-),男,广东陆丰人,在读博士研究生,研究方向为水稻抗病育种。E-mail:chougu@126.com
通讯作者:杨祁云(1966-),女,广东揭阳人,研究员,研究方向为水稻病害研究。E—mall:yangqy@gdppri.com;
陈志强(1956-),男,广东揭阳人,教授,研究方向为水稻遗传育种。E-mail:chenlin@8cau.edu.cn