摘要
目的探讨不同临床类型乙型肝炎患者HBV血清标志物及HBV DNA含量关系,为乙肝防治提供依据。方法 306例乙肝患者中,慢性肝炎104例、肝硬化126例、肝癌76例,采用酶联免疫吸附试验(ELISA)法检测血清HBV标志物(HBsAg、抗-HBs、HBeAg、抗-HBe、抗-HBc),用荧光定量PCR(FQ-PCR)检测HBV DNA,分析两者的关系。结果 306例患者小三阳(HBsAg阳性、抗-HBe阳性、抗-HBc阳性)检出率为47.06%(144/306),大三阳(HBsAg阳性、HBeAg阳性、抗-HBc阳性)检出率为20.26%(62/306);慢性肝炎组大三阳检出率、HBV DNA含量均高于肝硬化组、肝癌组(P均<0.01);慢性肝炎组小三阳检出率均低于肝硬化组、肝癌组(P均<0.01)。62例大三阳组患者HBV DNA含量高于144例小三阳组患者(P<0.05)。结论慢性肝炎患者HBV血清标志物以大三阳表现为主,肝硬化、肝癌患者以小三阳表现为主,慢性肝炎患者HBV DNA含量高于肝硬化、肝癌患者,HBV血清标志物及HBV DNA联合检测对乙型肝炎患者的临床诊断及治疗具有重要意义。
Objective To explore the relationship between hepatitis B virus（HBV） serum markers and content of HBV-DNA in patients with hepatitis B of various clinical types,and to provide evidence for prevention and treatment of hepatitis B. Methods Three hundred and six patients with hepatitis B were enrolled in the study, which including 104 cases of chronic hepatitis B, 126 cases of liver cirrhosis and 76 cases of liver cancer. HBV serum markers（ HBsAg,anti-HBs,HBeAg,anti-HBe,anti-HBc） were measured by enzyme immunoassay（ELISA） and the content of HBV-DNA was detected by fluorescence quantitative PCR（FQ-PCR） ,and their relationship was analyzed. Results Of 306 cases, patients with positive HBsAg,positive anti-HBe and positive anti-HBc accounted for 47.06% （144/306） while patients with positive HBsAg, positive HBeAg and positive anti-HBc accounted for 20.26% （62/306）. The detection rate of patients with positive I-IBsAg, positive HBeAg and positive anti-HBc, and the content of HBV DNA were significantly higher in chronic hepatitis group than those in other groups（ all P 〈 0.01 ） ;The detection rate of patients with positive HBsAg,positive anti-HBe and positive anti-HBc was significantly lower in chronic hepatitis group than that in other groups （all P 〈0.01）. The content of HBV DNA in 62 patients with positive HBsAg,positive HBeAg and positive anti-HBc was significantly higher than that in 144 patients with positive HBsAg, positive anti-HBe and positive anti-HBc （P 〈 0.05 ）.Conclusion HBV serum markers in patients with chronic hepatitis are dominated by positive HBsAg, positive anti-HBe and positive anti-HBe while in the patients with liver cirrhosis or hepatic cancer are dominated by positive HBsAg, positive anti-HBe and positive anti-HBc. The content of HBV DNA is higher in the patients with chronic hepatitis than that in the patients with liver cirrhosis or hepatic cancer. The combination detection of HBV serum markers and HBV DNA has great significance in the diagnosis and treatment of patients with hepatitis B.
出处
《广西医学》
CAS
2012年第10期1279-1281,共3页
Guangxi Medical Journal
基金
国家自然科学资金项目(30960326)
作者简介
广西中医药大学第一附属医院检验科，南宁市530023，E-mail：chenqingyan060@sina．com
陈钦艳，E—mail：chenqingyan060@sina．com
