摘要
[目的]研究葡萄籽多酚的超声波提取条件和抗氧化活性。[方法]以超声提取获得葡萄籽多酚,采用Fenton反应.OH体系研究抗氧化活性。[结果]最佳提取工艺条件为:超声提取60 min,浓度75%乙醇,料液比为1∶20。在最佳条件下,多酚得率为10.71%。葡萄籽多酚提取物对Fenton反应羟自由基清除的IC50为0.031 g/L,0.093 g/L时清除率达最大,最大清除率为87.12%。[结论]葡萄籽多酚超声提取具有良好的抗氧化活性。
[Objective] The research aimed to study the ultrasonic extraction and antioxidant activity of polyphenols extracted from grape seeds.[Method] Polyphenols of grape seeds was extracted by ultrasonic extraction and antioxidant activity was determined by scavenging effect on Fenton reaction ·OH radical with spectrophotometry.[Results] The optimum condition for the extraction of polyphenols were as follows： 60 min of ultrasonic time,75% of alcohol and 1∶ 20 of solid-liquid ratio.An extraction rate for the polyphenols from grape seeds was 10.71% under the optimum condition.The maxium scavening rate of 0.093 g/L extraction on ·OH was 87.12 % and IC50 reached 0.031 g/L.[Conclusion] The polyphenols from grape seeds obtained by ultrasonic extraction have good antioxidant activity.
出处
《安徽农业科学》
CAS
2012年第25期12341-12342,共2页
Journal of Anhui Agricultural Sciences
关键词
超声波
葡萄籽
多酚
提取
抗氧化
Ultrasonic
Grape seeds
Polyphenol Extraction
Antioxidantion
作者简介
张晓梅（1964-），女，四川宜宾人，实验师，从事化学方面的研究，E—mail：645885881@qq．com。
