摘要
采用油菜幼苗下胚轴作为外植体,分析不同品种、苗龄以及激素配比对愈伤组织出愈率的影响。结果表明:相同条件下,扬油6号的出愈率最高;扬油6号在5d苗龄、预培养3d以及加入0.1mg·L-1 2,4-D、1.0mg·L-1 6-BA、5.0mg·L-1 AgNO3条件下的愈伤组织诱导率最高。
In the past decades,the plant genetic engineering technology in improving the yield and quality of Brassica napus L.had been greatly developed.Establishing an efficient and stable regeneration system of explant is one of the important segments of genetic engineering breeding.In this study,hypocotyls of Brassica napus L.were used as the explant,and the callus induction ratio was analyzed under different genotypes,seedling ages and combinations of various hormones.The results showed that Yangyou 6 hypocotyl explant had the largest contribution on the callus formation under the same tissue culture conditions,and the best conditions of callus formation was the 5 d age seedling,pre-culturing for 3 d,culturing in the medium containing 0.1 mg·L-1 2,4-D,1.0 mg·L-1 6-BA and 5.0 mg·L-1 AgNO3.
出处
《扬州大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2012年第2期58-61,共4页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
国家自然科学基金资助项目(31171581)
江苏省研究生创新项目(CXZZ12-0897)
作者简介
杜坤(1980-),男,江苏南京人,扬州大学讲师,主要从事转基因油菜研究;联系作者:王幼平,E-mail:wangyp@yzu.edu.cn
