摘要
运用固相萃取纯化技术与高效液相色谱/二极管阵列检测器/电喷雾质谱联用技术,采用ZorbaxSB-C18色谱柱(250mm×4.6mm,5μm),甲醇-5%甲酸水溶液为流动相,流速为1.0mL/min,检测波长为520nm,以矢车菊素3-葡萄糖苷为对照,外标法测定了野生桑葚花色苷含量,并通过紫外扫描光谱和电喷雾质谱正离子碎片信息确定了花色苷的成分组成。结果表明:野生桑葚总花色苷含量为154.27mg/100g,含有的三种花色苷成分分别为矢车菊素3-葡萄糖苷、矢车菊素3-芸香糖苷和天竺葵素3-葡萄糖苷,其相对含量为67.52%、31.29%和1.06%。
To establish a method to determine anthocyanin content and composition in wild mulberry,solid phase extraction（SPE）and high performance liquid chromatography equipped with a DAD detector and electro-spray ionization mass spectrometry（HPLC/DAD/ESI/MS）were used.The analysis was carried on Zorbax SB-C18 column（250mm×4.6mm,5μm）.The mobile phase was aqueous 5% formic acid solution and methanol at the flow-rate of 1.0mL/min,the detection wavelength was 520nm.The content of gross anthocyanins was 154.27mg/100g by HPLC expressed as milligrams of cyanidin 3-glucoside equivalent per 100g of fresh weight.Three anthocyanins in wild mulberry were identified by UV-Vis and ESI＋-MS spectra which were cyanidin 3-glucoside,cyanidin 3-rutinoside and pelargonidin 3-glucoside,the relative content of which were 67.52%,31.29%,1.06% respectively.
出处
《食品工业科技》
CAS
CSCD
北大核心
2012年第15期307-310,共4页
Science and Technology of Food Industry
基金
公益性行业(农业)科研专项(201103037)
北京市属高等学校人才强教深化计划资助项目
北京市职业院校职教名师资助项目
关键词
野生桑葚
花色苷
固相萃取
高效液相色谱-质谱联用
wild mulberry
anthocyanin
solid phase extraction
high performance liquid chromatography-mass spectrometry（HPLC/MS）
作者简介
陈亮（1986-），男，博士生，主要从事天然产物的分离纯化研究。
通讯联系人
