摘要
目的:研究旱生香茶菜的化学成分。方法:采用多种色谱技术和方法进行分离,通过波谱分析鉴定化合物结构。采用MTT法进行人体肿瘤细胞毒体外活性实验。结果:从旱生香茶菜叶中分离并鉴定了10个三萜类化合物:齐墩果酸(1),乌苏酸(2),maslinicacid(3),2α-羟基乌苏酸(4),hederagenin(5),3β,23-dihydroxy-urs-12-en-28-oicacid(6),arjunolicacid(7),asiaticacid(8),2α,3α,24-trihydroxyolean-12-en-28-oicacid(9),2α,3α,24-trihydroxyurs-12-en-28-oicacid(10)。测试了10个化合物对3种(K562,MKN45,HepG2)人体肿瘤细胞株的细胞毒活性。结论:化合物1-10均首次从该植物中分离得到,并讨论了它们的生源关系。所有三萜化合物对上述三种人体肿瘤细胞株都没有细胞毒活性。
AIM： To investigate the chemical constituents of lsodon xerophilus. METHODS： The constituents were isolated by various chromatographic methods, and the chemical structures were elucidated on the basis of spectral analyses. Their cytotoxicity was evaluated by the MTT method in 96-well microplates. RESULTS： Ten triterpenes were isolated and identified as oleanolic acid （1）, ursolic acid （2）, maslinic acid （3）, 2α-hydroxyursolic acid （4）, hederagenin （5）, 3β, 23-dihydroxy-urs-12-en-28-oic acid （6）, arjunolic acid （7）, asiatic acid （8）, 2α, 3α, 24-trihydroxyolean-12-en-28-oic acid （9）, and 2α, 3α, 24-trihydroxyurs-12-en-28-oic acid （10）. All compounds were tested against three human tumor cell lines, K562, MKN45, and HepG2. CONCLUSION： All of the compounds were isolated from this plant for the first time. The proposed biosynthetic pathway of these triterpenes was discussed, All of the triterpenes showed noncytotoxic activity against the above-mentioned three human tumor cell lines.
出处
《中国天然药物》
SCIE
CAS
CSCD
2012年第4期307-310,共4页
基金
supported by the National Natural Science Foundation of China(No.21172024 to L.-M.Li)
the Key Project of Chinese Ministry of Education(No.212151)
the NSFC-Joint Foundation of Yunnan Province(No.U0832602 to H.-D. Sun)
the Major State Basic Research Development Program of China(Nos. 2009CB522300 and 2009CB940900)
the Science and Technology Program of Yunnan Province(Nos. 2008IF010 and 2008CD162)
the Major Direction Projection Foundation of CAS Intellectual Innovation Project(No.2010KIBA05 to J.-X.Pu)~~
关键词
旱生香茶菜
唇形科
三萜
生源途径
细胞毒活性
lsodon xerophilus
Labiatae
Triterpenes
Biosynthetic pathway
Cytotoxicity
作者简介
Corresponding author:李丽梅,Ph.D,Tel:86-28-68289083,E-mail:limeiligy@gmail.com.
