摘要
为探讨不同碳、氮源对甘蓝链格孢菌菌丝生长的影响,以从甘蓝品种W-14感病株上分离获得的菌种为供试材料,以该菌种在不同碳、氮源培养基上的菌落长势、直径、菌丝生长速率、生长指数、菌丝干重为测量指标,研究在基础培养基中添加13种常见氮源和6种常见碳源对甘蓝链格孢菌菌丝生长的作用,筛选出适宜该菌生长的营养物质。结果表明:在基础培养基中添加硝酸铵时,菌丝的生长速率及菌落直径较大,菌丝致密,其菌丝的生长指数最高为38.33,菌丝干重也较大;而在以淀粉为碳源的培养基上,菌丝的生长指数和菌丝干重最大,所以甘蓝链格孢菌菌丝生长的最佳氮源、碳源分别为硝酸铵和淀粉。
The research was to study the effect of different carbon sources and nitrogen sources on mycelial growth of Alternaria brassicicola in Cabbage.Cabbage varieties＂W-14＂isolated from acquired strains were used as test materials.Colony growth and diameter,mycelial growth rate,dry weight of mycelium from the strain in different carbon,nitrogen sources of culture medium were used as measurement index to study the function of 13 kinds of common species of nitrogen sources and 6 kinds of common carbon sources on the mycelial growth of Alternaria brassicicola in cabbage in order to select for the best Alternaria brassicicola growth nutrients.The results showed that： in the base medium with the addition of ammonium nitrate,the mycelium growth rate and colony diameter was higher,mycelial density,the hyphal growth index was the highest at 38.33.The dry weight of mycelium was larger.When starch was used as the carbon source of the culture medium,mycelial growth index and the dry weight of mycelium were the highest,therefore the ammonium nitrate was identified to be the best nitrogen source and starch was the best carbon source for the mycelial growth of Alternaria brassicicola.
出处
《作物杂志》
CAS
CSCD
北大核心
2012年第3期48-52,共5页
Crops
基金
国家科技支撑计划(2006BAD01A7-2-05)
关键词
甘蓝
链格孢菌
碳氮源
菌丝生长
Cabbage
Alternaria brassicicola
Nitrogen and carbon sources
Mycelial growth
作者简介
王宁宁，硕士研究生，研究方向为蔬菜育种学
王超为通讯作者，教授，从事甘蓝育种研究
