摘要
用常规鉴定和Biolog快速鉴定2种方法对分离得到的3株产脂肪酶菌株进行了分类鉴定。根据其菌落、菌体及生理生化特征,3株产脂肪酶菌株中CDCH110、CDCH111属于不动杆菌属,CDCH112属于假单胞菌属。Biolog快速鉴定结果显示:CDCH110为溶血不动杆菌;CDCH111为醋酸不动杆菌;CDCH112为产碱假单胞菌。通过对比分析,结果表明:2种方法得到的结果一致,但常规方法只能鉴定到属,Biolog快速鉴定可以鉴定到种;Biolog快速鉴定法具有快速、准确等优点。
Three microbe with lipase activity, isolated tional identification methods and Biolog Microstation Sy by ste our laboratory, were identified by conven- m. The results from conventional identifica- tion showed that microbe CDCH110, CDCH111 all belonged to Acinetobacter sp. , and CDCH112 be- longed to Pseudomonas sp.. It indicated that CDCHll0 was Acinetobacter calcoaceticus, CDCHll 1 was Acinetobacter haemolyticus, and CDCH111 was Pseudomonas alcaligenes with Biolog Microstation Sy of stem. The results from two methods showed a well consistency. However, it could only get the genus microbe by the way of conventional identification methods, and the Biolog system could get the spe- cies. In addition, Biolog Microstation System has high accuracy
出处
《重庆理工大学学报（自然科学）》
CAS
2012年第5期24-27,共4页
Journal of Chongqing University of Technology：Natural Science
关键词
菌种鉴定
细菌
常规鉴定
species identification
bacteria
conventional identification
作者简介
叶姜瑜（1963-），男，四川青川人，博士，副教授，主要从事环境微生物及微生物生态研究。
