摘要
提出了液相色谱-串联质谱法测定食品中8种合成色素柠檬黄、苋菜红、胭脂红、日落黄、诱惑红、亮蓝、赤藓红和偶氮玉红的含量。固体(半固体)样品经乙醇-氨水-水(70+1+29)溶液溶解,所得滤液烘干后用水溶解,经Waters Atlantis dC18色谱柱(2.1 mm×150 mm,5μm)分离,用甲醇和10 mmol.L-1乙酸铵溶液以不同体积比混合进行梯度洗脱,采用电喷雾正离子模式串联质谱检测。8种合成色素的质量浓度均在50μg.L-1以内与其峰面积呈线性关系,检出限(3S/N)在0.003～0.020 mg.kg-1之间。方法应用于食品样品中8种合成色素的测定,回收率在83.0%～112.0%之间,测定值的相对标准偏差(n=6)均小于5%。
LC MS/MS was applied to the determination of 8 kinds of synthetic colorants, including tartrazinein, amaranth, ponceau 4R, sunset yellow, fancy red, EDS, food red 14 and azorubine in food. The solid or semi-solid samples were dissolved with C2 H5OH-NH3 （aq.） and H2 O （ 70 ＋ 1 ＋ 29） solution, and the filtrate liquor was evaporated to dryness. The residue was dissolved with water and used for LC-MS/MS determination, using Waters Atlantis dC18 column （2. 1 mm×150 mm, 5 μm） as chromatographic column, and a mixture of methanol and 10 mmol . L^-1 ammonium acetate with the different ratios as mobile phase for gradient elution. Positive electrospray ionization mode was used in the MS/MS detection. Linear relationships between values of peak area and mass concentration of the 8 synthetic colorants were obtained in the range within 50μg . L^- 1 with their detection limits （3S/N） in the range of 0. 003- 0. 020 mg . kg^-1. The proposed method was used to the determination of the 8 synthetic colorants in food, giving values of recovery in the range of 83. 0%-112.0% with RSD＇s （n=6） less than 50%.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2012年第4期445-448,共4页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
作者简介
王登飞（1976-），男，福建福清人，硕士，工程师。主要从事食品安全检测工作。E-mail：wdfll7@sohu．com
