摘要
为了探讨新城疫病毒(NDV)对雏鸵鸟肾脏超微结构的影响。以45天雏鸵鸟为试验动物,分正常组和攻毒组(皮下注射鸡新城疫标准毒株F48E9);利用透射电镜(TEM)技术,观察攻毒组鸵鸟肾脏的超微结构。结果表明:攻毒组鸵鸟肾脏肾小体和肾小管均有明显变化,肾小体内皮细胞肿胀,囊腔缩小,滤过屏障受损;肾小管以空泡变性为主,上皮细胞肿胀,微绒毛减少或断裂,胞核固缩、染色质呈块状边集于核膜下或溶解减少,胞浆内可见大量溶酶体,线粒体嵴断裂、空泡化。结果提示,鸡源NDV对雏鸵鸟肾脏高度敏感,可引起肾脏组织明显的病理组织学变化。
In order to study the effects of Newcastle disease viruses (NDV) on kidney ultrastructure in ostrich chicks,ostrich chicks aged 45 days were divided into normal group and challenge group at random.The methods of transmission electron microscopy (TEM) were used to study the kidney ultrastructure of ostrich chicks in challenge group.The results showed that the obvious morphologic change of renal corpuscle and renal tubule was observed in the challenge group,including swelling of endothelial cell,shrink of capsular space,damage of filtration barrier in renal corpuscle and vacuolar degeneration in renal tubule,with swelling of epithelial cell,ruptured and decreased of microvillus,nuclear pyknosis,the chromatins distributed along the nuclear envelopes or dissolve decrease,increased number of lysosomes,mitochondrial cristae rupturing and maculated mitochondrial.The results indicated that the kidney in ostrich was highly sensitive with NDV,which could caused significant change on histopathology of the kidney.
出处
《中国农学通报》
CSCD
2012年第11期61-65,共5页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金资助项目"禽类脑脊髓传导径的起源及细胞构筑"(39970547)
国家自然科学基金资助项目"鸵鸟脑干神经核团的分布和细胞构筑的研究"(30471249)
四川农业大学博士专项(00270403)
关键词
鸵鸟
肾脏
新城疫
超微结构
Ostrich chicks
Kidney
New castle disease
ultrastructure
作者简介
唐丽,女,1979年出生,陕西洛南人,讲师,博士,主要从事禽类形态学研究。通信地址:625014四川省雅安市雨城区四川农业大学动物医学院,Tel:0835—2885754,E-mail:tangyimingtt@163.com。
通讯作者:彭克美,男,1953年出生,湖南长沙人,教授,博士生导师,主要从事神经科学和发育生物学研究。通信地址:430070湖北省武汉市华中农业大学动物医学院,Tel:027.87286970:E-mail:kmpeng@sohu.com.