摘要
目的研究碧云喷雾剂对2,4-甲苯二异氰酸酯(TDI)致豚鼠变应性鼻炎的治疗作用。方法以TDI为致敏原,建立豚鼠变应性鼻炎模型,将实验动物随机分为正常对照组、模型对照组、碧云喷雾剂组、酮替芬组。正常对照组和模型对照组给予相应容量0.9%氯化钠溶液,碧云喷雾剂组给予碧云喷雾剂96 mg.kg-1(相当于临床等效剂量的2倍),酮替芬组给予酮替芬0.07 mg.kg-1。连续给药7 d。观察各组鼻部过敏症状并评分,对鼻黏膜组织做病检,并对嗜酸性粒细胞(EOS)、肥大细胞(MC)计数。结果正常对照组、模型对照组、碧云喷雾剂组、酮替芬组鼻部过敏症状评分分别由治疗前(1.17±0.63),(7.05±1.24),(7.56±1.05),(7.69±0.96)分,降为(1.11±0.54),(7.33±0.67),(4.92±0.96),(5.14±0.77)分,碧云喷雾剂明显降低豚鼠鼻部过敏症状评分(P<0.01)。鼻黏膜组织病理变化得到明显改善,正常对照组、模型对照组、碧云喷雾剂组、酮替芬组EOS分别为(1.68±0.85),(9.13±1.68),(4.51±2.25),(3.59±1.77)个;MC分别为(2.23±1.26),(21.03±5.03),(14.05±6.88),(13.14±5.41)个,脱颗粒MC分别为(1.32±1.03),(13.64±5.47),(8.49±2.37),(6.06±2.55)个,碧云喷雾剂下调EOS、MC作用明显(P<0.01)。结论碧云喷雾剂对豚鼠TDI致变应性鼻炎有一定的治疗作用。
Objective To study the effects of Biyun spray on allergic rhinitis（AR） induced by 2,4-tolunen diisocyanate（TDI） in guinea pigs.Methods TDI was selected as sensibiligen and administered intranasally to develop the AR model.The experimental guinea pigs were randomly divided into 4 groups： control group,model control group,Biyun spray group,positive control（ketotifen fumarate） group.Corresponding volume of 0.9% sodium chloride solution was administered to the control and model control groups.In the Biyun spray group,Biyun spray was administered at a dose of 96 mg·kg-1,which was twice the clinical equivalent dose.Ketotifen fumarate was administered to positive control group at a dose of 0.07 mg·kg-1.Symptoms were observed and the nasal symptom score of AR was determined.The pathological changes of nasal mucosa tissue were observed under a microscope.Eosinophilic granulocytes（EOS） and mast cells（MC） were counted.Results Among the control,model control,Biyun spray,and positive control groups,the nasal symptom scores of AR were decreased from（1.17±0.63）,（7.05±1.24）,（7.56±1.05）,（7.69±0.96） to（1.11±0.54）,（7.33±0.67）,（4.92±0.96）,（5.14±0.77）,respectively after the treatments.Biyun spray significantly decreased the nasal symptom score of AR（P0.01）.Pathological changes of nasal mucosa tissue were improved obviously.Among the control,model control,Biyun spray,and positive control groups,the EOS number was（1.68±0.85）,（9.13±1.68）,（4.51±2.25） and（3.59±1.77）,respectively;the MC number was（2.23±1.26）,（21.03±5.03）,（14.05±6.88） and（13.14±5.41）,respectively;the degranulation MC number was（1.32±1.03）,（13.64±5.47）,（8.49±2.37） and（6.06±2.55）,respectively.Biyun spray significantly improved the pathological changes of nasal mucosa with EOSs and MCs being decreased significantly（P0.01）.Conclusion Biyun spray is effective in treating allergic rhinitis in guinea pigs.
出处
《医药导报》
CAS
北大核心
2012年第4期414-416,共3页
Herald of Medicine
基金
湖南省中医药科研项目基金(2010056)
湖南省重点学科建设资助项目(1007)
作者简介
贺卫和（1972-），男，湖南双峰人，副教授，硕士，研究方向：中药有效成分药效学研究。电话：0731—88458238，E—mail：heweihe2003@yahoo．com．cn。
通讯作者王志琪（1976-），女，天津人，讲师，博士，研究方向：天然药物安全性与有效性研究。电话：（0）13017285185，E-mail：wzqx2004@126．com。
