摘要
为了研究乌头碱的生物转化规律,建立了乌头碱及其代谢物分离与鉴定的液相色谱-电喷雾离子阱质谱(LC-ESI-ITMS)方法。首先利用体外代谢法得到大鼠肝微粒体S9组分中乌头碱及代谢产物混合体系,然后利用LC-ESI-ITMS联用技术对各代谢物进行分析,得到乌头碱代谢物的[M+H]+分子质量信息,进一步结合多级串联质谱(LC/MSn)分析结果获得结构信息,对代谢物进行鉴定,从而推测代谢物结构,并结合大鼠尿中乌头碱及其代谢物的分析结果,得到乌头碱的生物转化规律。本方法在大鼠肝微粒体S9组分中鉴定得到8种体外代谢产物。
A method applying liquid chromatography-electrospray ionization-ion trap mass spectrometry （LC-ESI-ITMS） for the simultaneous analysis of Aconitine and its in-vitro metabolites is described. Aconitine was incubated with mouse liver microsome S9 fraction firstly, and then the incubation sample was analyzed by LC-ESI-ITMS in full-scan mode. Aconitine and its metabolites were identified from the determination of molecular ions. The metabolites detection were confirmed from tandem mass spectrometry （MSn） analysis. Comparing with the analysis of urine, 8 metabolites were identified in the mouse liver S9 fractions by comparing with the analysis of urine of rat oral with Aconitine and referring to the literature reports.
出处
《质谱学报》
EI
CAS
CSCD
北大核心
2012年第2期65-73,共9页
Journal of Chinese Mass Spectrometry Society
基金
香港浸会大学项目(FRG/08-09/II-01)资助
关键词
液相色谱-电喷雾离子阱质谱
肝微粒体
乌头碱
代谢
liquid ehromatography-eleetrospray ion trap mass spectrometry
mouse liver S9 fractions
Aconitine
in-vitro metabolism
作者简介
陈学国（1977-），男（汉），副教授，从事毒物、毒品分析。E—mail：chenxg@dicp．ac．cn
通信作者：蔡宗苇（1962～），男（汉），教授，从事药物、环境污染物分析。E-mail：zwcai@hkbu．edu．hk
