摘要
【目的】寻找对斑玉蕈菌丝生长有促进作用的无机盐、维生素及植物生长调节剂,为斑玉蕈液体菌种的进一步开发与利用提供参考。【方法】以斑玉蕈菌株HM-2为材料,向培养基中加入不同种类及质量浓度的无机盐、维生素和植物生长调节剂,于(23±1)℃、空气相对湿度70%～75%的条件下培养12d后测定菌落直径,研究上述物质对斑玉蕈菌丝生长的影响。【结果】与不添加任何无机盐的培养基相比,0.5～2.0g/L的MgSO4、NaCl和KCl对斑玉蕈菌丝生长均有一定的促进作用,且均以1.5g/L时促进作用最大,菌落直径分别达到81.04,78.93和80.29mm;而0.5～2.0g/L的CaCl2、KH2PO4和0.01～0.04g/L的ZnSO4对斑玉蕈菌丝的生长具有不同程度的抑制作用。与不添加任何维生素的培养基相比,2.0～10.0mg/L的烟酸、叶酸、VB1和VB2对斑玉蕈菌丝生长均有一定的促进作用,以2.0mg/L叶酸的促进作用最大,菌落直径达到67.76mm;而2.0～10.0mg/L的VB6对斑玉蕈菌丝生长无明显的促进和抑制作用。与不添加任何植物生长调节剂的培养基相比,2.0～10.0mg/L的IBA、GA3和NAA对斑玉蕈菌丝生长均有极显著的促进作用,以2.0mg/L IBA的促进作用最大,菌落直径达到69.33mm;而2.0～10.0mg/L的KT与6-BA对斑玉蕈菌丝生长的促进作用相对较弱。【结论】在培养基中添加适量的MgSO4、NaCl、KCl、烟酸、叶酸、VB1、VB2、IBA、GA3和NAA,可促进斑玉蕈菌丝的生长。
[Objective] The research was made to look for several different kinds of inorganic salts,vi- tamins and plant hormones which have definite promotion on the mycelial growth of Hypsizigus mar- moreus （Peck） Bigelow,in order to provide reference information for development and application of H. rnarmoreus （Peck） Bigelow liquid cultivation. [Method] H. marmoreus （Peck） Bigelow HM-2 was taken as test material, which was cultivated in culture mediums with different kinds of inorganic salts, vitamins and plant hormones under the temperature of （23＋1） ℃,RH 70%-75% ,and then the colony sizes of the dif- ferent culture mediums were measured after twelve days to research the effect of different kinds of inorgan-ic salts,vitamins and plant hormones with different concentrations on the growth of H. rnarmoreus （Peck） Bigelow mycelium. [Result] Compared with no inorganic salts applicated culture mediums,0.5-2.0 g/L magnesium sulfate, sodium chloride and potassium chloride have definite promotion on the mycelium growth of H. marmoreus （Peck） Bigelow,the biggest colony size of H. marmoreus （Peck） Bigelow is 81.04 mm,78.93 mm and 80.29 mm respectively in the concentration of 1.5 g/L,but 0.5-2.0 g/L calcium chlo- ride,potassium dihydrogen phosphate and 0.01-0.04 g/L zinc sulfate have different degrees of inhibition on the mycelium growth of H. marmoreus （Peck） Bigelow. Compared with no vitamins applicated culture mediums,2.0-10.0 mg/L Nicotinic acid,folic acid,Vm and VB2 promote the mycelium growth of H. mar- moreus （Peck） Bigelow,the biggest colony size of H. marrnoreus （Peck） Bigelow is 67.76 mm in the folic acid concentration of 2.0 mg/L,but VB6 has neither promotion nor inhibition on the mycelium growth of H. marmoreus （Peck） Bigelow. Compared with no plant hromones applicated culture mediums, 2.0- 10.0 mg/L IBA, GA3 and NAA of plant hormones have prominent promotion on the mycelium growth of H. marmoreus （Peck） Bigelow,the biggest colony size of H. marmoreus （Peck） Bigelow is 69.33 mm in the IBA concentration of 2. 0 mg/L, but 2. 0- 10. 0 mg/L KT and 6-BA have less effect on the mycelium growth of H. marmoreus （Peck） Bigelow in comparion to the other plant hormones. [Conclusion] Adding moderate concentrations of magnesium sulfate, sodium chloride, potassium chloride, nicotinic acid, folic acid,Vm ,VB2 ,IBA,GA3 and NAA could enhance the mycelium growth of H. marmoreus （Peck） Bigelow.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2012年第3期158-162,共5页
Journal of Northwest A&F University(Natural Science Edition)
基金
陕西省“13115”科技创新工程重大科技专项(2010ZDKG-72)
关键词
斑玉蕈
无机盐
维生素
植物生长调节剂
Hypsizigus marmoreus （Peck） Bigelow inorganic salts vitamins plant hormones
作者简介
吴秀珍（1986-），女，山东济宁人，在读硕士，主要从事微生物资源与利用研究。E-mail：wuxiuzhen0910@163．com
杜双田（1961-），男，陕西扶风人，副教授，主要从事食用与药用真菌研究。E-mail：dst6107@126．com
