摘要
建立了气相色谱-质谱(GC-MS)法同时测定化妆品中24种过敏原的分析方法。样品经甲醇超声提取,以选择离子模式(SIM)进行定性及定量分析。24种过敏原在质量浓度0.2～100 mg/L范围内呈良好的线性关系,相关系数r2﹥0.995,检出限为2～10 mg/kg,平均回收率为80%～104%,相对标准偏差为1.8%～9.3%。该方法快速、准确、灵敏度高。通过对77名患者使用的150份可疑致敏化妆品测试发现,24种过敏物质的阳性检出率为78%,其中至少有1种过敏物质含量大于10 mg/kg的样本占阳性样本的81.2%。71名过敏患者(92.2%)使用的化妆品中检出致敏物;其中至少有一种过敏物质的检出值大于10 mg/kg的占90.1%。结果显示,24种过敏物质是化妆品接触性皮炎的主要致敏原。
A simultaneous determination method was developed for the reliable screening, quantifica- tion and confirmation of 24 allergens in cosmetics by gas chromatography -mass spectrometry（ GC - MS）. The 24 allergens were extracted from cosmetics with methanol, then determined by GC - MS with the external standard method under the selected ion mode （SIM）. The calibration curves showed good linearities in the range of 0.2 - 100 mg/L with correlative coefficients （ r^2 ） larger than 0. 995. The detection limits were in the range of 2 - 10 mg/kg, and the average recoveries were 80% - 104% with relative standard deviations of 1.8% -9.3% . The method was rapid, accurate and sen- sitive. The positive detection rate of 24 allergens in 150 samples of 77 patients using cosmetics was 78% , and there was at least one allergen measured value above 10 mg/kg in 81.2% of the positive samples. 24 allergens in the cosmetics used by 71 patients were detected out and the detection rate was 92.2% , wllich at least one allergen measured value above 10 mg/kg was 90. 1% . 24 allergens are the major allergens in cosmetic contact dermatitis.
出处
《分析测试学报》
CAS
CSCD
北大核心
2012年第3期261-266,共6页
Journal of Instrumental Analysis
基金
国家质检总局科技计划项目(2009IK160)
关键词
气相色谱-质谱
化妆品
过敏原
接触性皮炎
gas chromatography - mass spectrometry
cosmetics
allergen
contact dermatitis
作者简介
焦红，研究员，研究方向：食品和化妆品实验室质量控制和安全评价，Tel：13903053501，E-mail：jhciql228@163．com
