摘要
目的观察猪急性颅高压损伤模型的病理生理变化及超微结构改变。方法选用中型猪5只,采用微量泵注射自体动脉血法建立急性颅高压损伤控制模型,模型制备过程中及制备后2 h持续监测颅内压、平均动脉压变化,注血后2 h行头部CT扫描,24 h取脑行大体切片,苏木精-伊红染色光镜观察组织学病理改变、超微电镜观察受损神经元的超微结构改变。结果注血后颅内压、平均动脉压均明显上升,最高分别达(51.2±2.5)mmHg和(152.7±12.8)mmHg,颅内压升至约50 mmHg时,出现呼吸、心率改变;头部CT扫描、大体切片均显示脑内血肿形成;电镜检查显示血肿区神经元肿胀,胞质内细胞器数量减少,线粒体肿胀,嵴排列不整齐,粗面内质网颗粒扩张,血管内皮细胞肿胀,基膜不完整,血管周围可见明显水肿带。结论采用微量泵注射自体动脉血法可建立稳定、可靠的猪急性颅高压损伤控制模型。
Objective To observe the pathophysiological and ultrastructural changes in the porcine model of acute intracranial hypertension brain injury.Methods Autologous arterial blood was injected into the brain in 5 middle-weighted pigs by micro-pump for establishing the brain injury model due to acute intracranial hypertension.And the change of intracranial pressure（ICP） and mean arterial pressure（MAP） were monitored.CT scanning was performed 2 h after blood injection.After HE staining,histological changes were observed under light microscope and ultrastructural changes under electron microscope.Results ICP and MAP significantly increased after the blood injection,and the peak reached 51.2 ± 2.5 mmHg and 152.7 ± 12.8 mmHg respectively.The porcine respiration and heart rate changed when the ICP increased to about 50 mmHg.CT scanning and pathological examination all showed the brain hematoma formed.Electron microscope examination showed that the neuronal swelling in hematoma area,decrease of the organelle number in cytoplasm,mitochondrial swelling and irregular arrangement of mitochondria cristae,enlargement of the rough endoplasmic reticulums,swollen vascular endothelial cells,the incomplete basement membrane,and obvious edema zone around vessels.Conclusions Autologous arterial blood injection by micro-pump can establish a stable control model of acute intracranial hypertension brain injury in pigs.
出处
《中国微侵袭神经外科杂志》
CAS
2012年第3期131-133,共3页
Chinese Journal of Minimally Invasive Neurosurgery
基金
全军"十一五"医药卫生科研基金(编号:06Z017)
关键词
颅内高压
损伤控制神经外科
模型
动物
猪
intracranial hypertension
damage control neurosurgery
models
animal
pig
