摘要
【目的】以免疫信息学和反向疫苗学为理论根据,应用生物信息学相关软件和方法,对BVDV结构蛋白(Structural Protein,SP)的二级结构的不同方面及其B细胞表位进行了预测,综合评价了BVDV结构蛋白的抗原特异性细胞表位,并计算出一些潜在的抗原位点。【方法】利用RT-PCR扩增法获得牛病毒性腹泻病毒结构蛋白序列,通过克隆、测序分析结构蛋白基因的基因组序列和蛋白序列,采用DNAStar Protean软件对结构蛋白的二级结构、可塑性、亲水性、表面可及性及抗原指数等参数进行综合分析,并预测其B细胞的抗原表位分布。【结果】P14、gp48和gp53蛋白含有的细胞优势抗原表位较多,有的甚至有可能成为优势抗原表位或对优势抗原表位有协同作用。【结论】与已鉴定的B细胞抗原表位相比,试验所用方法预测的结果有较高的准确度,是一种较为先进的表位研究方法。
[ Objective ] On the basis of immunological bioinformatics and reverse vaccinology, the second- ary structure of the BVDV structural protein （SP） and its B lymphocyte epitopes with specificity of antigen were predicted and evaluated comprehensively, and some potential antigenic determinants were worked out from the SP gene sequence of the strain with related software and methods of bioinformatics. [ Method ] The structural protein（SP） gene of the bovine viral diarrhea virus （BVDV） was amplified by RT -PCR and then sequenced. The secondary structure, flexibility, hydrophilicity, accessibility and antigenicity of pl were ana- lyzed by using DNA Star Protean software, and the B cell epitopes were predicted. [ Result ] The results showed that there were several domains in P14, gp48 and gp53 which belonged to predominant B cell epitopes and a few of B cell epitopes were in gp25. [ Conclusion] Compared with the epitopes that have been pub-lished, the results predicted by this method had high reliability and this method was a more advanced epitope research approach.
出处
《新疆农业科学》
CAS
CSCD
北大核心
2012年第2期310-317,共8页
Xinjiang Agricultural Sciences
基金
新疆兵团青年创新资金项目(2010JC22)
国家自然科学基金项目(30960286
30960281)
新疆生产建设兵团博士资金课题(2007JC15)
关键词
牛病毒性腹泻病毒
结构蛋白
B细胞表位
二级结构
反向疫苗学
Bovine Viral Diarrhea Virus （BVDV）
structural protein
B cell epitope
secondary strue- ture
reverse vaecinology
作者简介
何延华（1981-），男，甘肃山丹人，助理研究员，研究方向为寄生虫分子生物学和分子病毒学，（E—mail）yanhuahe2008@yahoo.com.cn
通讯作者：王新华（1957-），男，陕西人，研究员，研究方向为瘟病毒单克隆抗体及寄生虫分子生物学，（E-mial）wangxihua5751@163.com
