1Rappuoli R. Reverse vaccinology, a genome-based approach to vaccine development[J]. Vaccine,2002,19:2 688-2 691.
2Grandi G. Antibacterial vaccine design using genomics and proteomics [J ]. Trends Biotechnol, 2001, 1 9:181-188.
3Altschul S F,Madden T L,Schaffer A A,et al. Gapped BLAST and PSI-BLAST:a new generation of protein database search program[J]. Nucleic Acids Res,1997,25:3 389-3 402.
4Altschul S F,Gish W,Miller W,et al. Basic local alignment search tool[J ]. J Mol Biol, 1990,215: 403-410.
5Claros M G,Brunak S,von Heijne G. Prediction of N-terminal protein sorting signals[J]. Curr Opin Struct Biol, 1997,7: 394-398.
6Nielsen H, Engelbrecht J, Brunak S, et al. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites[J]. Protein Eng, 1997,10: 1-6.
7Rost B,Fariselli P,Casadio R. Topology prediction for helical transmembrane protein at 80 % accuracy[J ]. Protein Sci, 1996,5:1 704-1 718.
8Rost B. PHD:predicting one-dimensional protein structure by profile-based neural networks [J]. Methods Enzymol, 1996,266:525-539.
9Struvve M. Carboxy-terminal phenylalanine is essential for the correct assembly of a bacterial outer membrane protein[J]. J Mol Biol, 1991,218:141-148.
10Havashi S,Wu H C. Lipoproteins in bacteria[J]. J Bioenerg Biomembr, 1990,22: 451-471.
6Vivona S, Gardy J L, Ramachandran S, et al. Computer-aided bio- technology: from immuno-informatics to reverse vaccinology [ J ]. Trends in Biotechnology, 2007, 26 (4) : 90-200.
7Davies M N, Flower D R. Harnessing bioinformatics to discover new vaccine [ J]. Drug Discovery Today, 12(9-10) : 389-395.
8Mora M, Veggi D, Santini L, et al. Reverse vaccinology [J]. Drug Discover Today, 2003, 10 (8) : 459-464.
9Xiang Z S, He Y Q. Vaxign: a web-based vaccine target design pro- gram for reverse vaccinology [ J]. Procedia in Vaccinology, 2009, 1 ( 1 ) : 23-29.
10Liang X, Teng A, Braun D M, merase chain reaction (TAP) : sing genome sequence data [ J ] ( 5 ) : 3593-3598. et al. Transcriptionally active poly- high throughput gene expression u- Gene Therapy Systems, 2002, 277.
