摘要
为分析附红细胞体感染后Th17细胞所发挥的免疫功能。采用RT-PCR及荧光抗体双标的方法检测小鼠脾脏IL-17的表达变化。结果显示:小鼠感染附红细胞体后,IL-17表达水平显著增高,提示Th17细胞在附红细胞体感染后发挥了重要的免疫作用,为该病的临床诊断、治疗及预防提供了理论依据。
To investigate the immunologic function of Thl7 cells for the mice infected with Eperythrozoon (Mycoplasma suis), the RTPCR assay and the fluorescence antibody technique were used to detect the variation of interleukin-17(IL-17) in mousey spleen tissue. The results showed that the IL-17 expression level increased obviously when the mice infected with Eperythrozoon. It prompted that the Th17 ceils participated in the immunoregulation, and played an important role in inflammation which the mice infected by Mycoplasma suis. Accordingly, this experimental research had offered some theoretical bases for the diagnosis, therapy and prevention about Eperythrozoonosis.
出处
《中国农学通报》
CSCD
2012年第5期62-66,共5页
Chinese Agricultural Science Bulletin
基金
辽宁省科技基金资助项目"附红细胞体和出血性大肠杆菌感染BALB/c小鼠模型的建立及对免疫功能影响的研究"(2006408002-6)
作者简介
杨蕾芳,女,1984年出生,河南新乡人,在读硕士。通信地址:121001辽宁省锦州市松坡路3段40号辽宁医学院,Tel:0416-4673585,E-mail:yanglfl63@sina.com。
通巴彩风,女,1951年出牛,辽宁锦州人,教授,差要从事人兽共患病研究。通信地址:121001辽宁省锦州市松坡路3段40号辽宁医学院,Tel:0416-4673585,Email:bacaifeng@163.com。