摘要
优选最佳白头翁皂苷B4提取与分离工艺。本试验采用不同萃取工艺分离白头翁皂苷;采用高效薄层色谱法、高效液相色谱法,通过比较得率、白头翁皂苷B4含量等,考察提取分离工艺对白头翁皂苷的影响。结果:工艺Ⅱ优于工艺Ⅰ。工艺Ⅱ不仅提取得率高,且皂苷B4集中在正丁醇部分。本项研究对白头翁皂苷提取分离工艺的选用提供了试验依据。
To study extraction and separation technology of pulchinenoside, pulsatillae was extracted by different abstraction technologyies. Pulchinenoside B4 content and yield were detected by HPLC and HPTLC. The results showed that technology Ⅱ is better than technology 1. Pulchinenoside B4 with high yield can be obtained by this technology Ⅱ and Pulchinenoside B4 was concentrated in n -butanol part. This research provides a test basis for B4 separation and purification.
出处
《安徽科技学院学报》
2011年第5期42-46,共5页
Journal of Anhui Science and Technology University
基金
安徽科技学院大学生创新课题
安徽科技学院自然科学研究项目(ZRC2011294)
安徽省中药材产业技术研发中心基金资助
作者简介
宋科标(1988-),男,本科生,主要从事中药提取与制备研究。通讯作者:时维静,教授,E-mail:shiwj80@yahoo.corn.cn。