摘要
基于Cytb基因,采用直接测序的方法,对阿拉斯加鳕鱼ITS1、大西洋庸鲽ITS2、牙鳕ITS3和欧洲黄盖鲽ITS4 4种鱼进行初步鉴定。结果表明,ITS1与Theragra chalcogramma(DQ356946)序列同源性达99%;ITS2与Hippo-glossus hippoglossus(EU492151)序列同源性达98%;ITS3与Merlangius merlangus(AY946299)序列同源性达98%;ITS4与Limanda limanda(EU492119)序列同源性达99%。本研究又对50份样品进行鱼种真实性鉴定,结果发现大部分样品与产品包装上的名称相符。
In this study Theragra chalcogramma, Hippoglossus hippoglossus, Merlangius merlangus and Limanda limanda were identified by sequence method, based on Cytb gene. The preliminary results showed that ITS1 was similar with Theragra chaleogramrna (DQ356946) by 99%, ITS2 was similar with Hippoglossus hippoglossus (EU492151) by 98%, ITS3 with Merlangius merlangua (AY946299) by 98%, ITS4 with Limanda limanda (EU492119) by 99%. We also detected 50 samples about fish authenticity identification, and testing results also showed that most samples are consistent with their name.
出处
《华北农学报》
CSCD
北大核心
2011年第B12期9-12,共4页
Acta Agriculturae Boreali-Sinica
作者简介
张洪沂(1982-),女,河北人,硕士,主要从事食品生物技术的研究。
通讯作者:柴平海(1965-),男,山西人,硕士,高级工种师。主要从事与食品有关的检测方法的研究。