摘要
以怀地黄花药为外植体,研究花药发育时期和激素对怀地黄花药愈伤组织诱导率和分化率的影响。结果表明:怀地黄花药在4℃条件下预处理3d后接种在含有2,4-D 2.0mg/L、6-BA 0.5mg/L和KT 2.0mg/L的MS培养基上对花药愈伤组织诱导最有利,而最适的愈伤组织分化培养基为MS+IAA 0.5mg/L+6-BA 1.0mg/L。
Taking the anthers from Rehmannia glutinosa Libosch as explants, several factors such as the stage of anther and different hormone treatments were studied on anther callus induction and shoot differentiation induction. The results showed that the optimum callus induction could be obtained by inoculation on MS culture medium containing 2,4-D 2. 0 mg/L,6-BA 0, 5 mg/L and KT 2.0 mg/L after storage at 4℃ for 3 d, and the optimums shoot differentiation medium was MS+IAA 0. 5 mg/L+6-BA 1.0 mg/L.
出处
《北方园艺》
CAS
北大核心
2012年第4期128-130,共3页
Northern Horticulture
基金
国家高技术研究发展计划"863"项目子课题资助项目(2006AA100109)
河南省教育厅自然科学研究计划资助项目(2009B180016)
河南省教育厅自然科学研究计划资助项目(2011B180035)
河南师范大学青年科学基金资助项目
关键词
怀地黄
花药培养
植株再生
Rehmannia glutinosa Libosch
anther culture
plant regeneration
作者简介
第一作者简介:周春娥(1977-),女,山西寿阳人,硕士,讲师,现从事植物分子与细胞育种研究工作。E-mail:041106@htu.cn。