摘要
目的 探讨红景天苷原料药对全脑缺血再灌注大鼠脑水肿和神经功能的影响.方法 100只Sp rague-Dawley大鼠随机分为假手术组、缺血再灌注组以及红景天苷12、24和48 mg/kg组,每组20只；各组再分为6h、24 h、72 h和7 d组,每组5只.四血管闭塞法建立大鼠全脑缺血模型.各组均在模型制作后即刻开始灌胃给药,连续7d.干湿重法检测脑水含量.采用神经功能缺损量表（neurologic deficit score,NDS）评价神经功能.结果 缺血再灌注组和红景天苷组在模型制作后均出现显著的神经功能缺损,并随着时间推移逐渐减轻.各红景天苷组神经功能缺损均较相应时间点缺血再灌注组显著改善（P均＜0.05）,且呈剂量依赖趋势；模型制作后72 h和7d时红景天苷48 mg/kg组神经功能缺损较红景天苷12 mg/kg组和24 mg/kg组显著改善（P均＜0.05）.缺血再灌注组和红景天苷组脑含水量均在制模后6h开始升高,72 h时达高峰,显著高于假手术组（P均＜0.05）.红景天苷组在模型制作后24 h和72 h时脑含水量显著低于缺血再灌注组（P均＜0.05）,呈剂量依赖趋势,到模型制作后7d时,红景天苷48 mg/kg组脑含水量已接近假手术组.结论 红景天苷能显著减轻全脑缺血再灌注大鼠脑水肿和改善神经功能,具有神经保护作用.
Objective To investigate the effect of salidroside on brain edema and neurological function in global cerebral ischemia-reperfusion injury in rats.Methods A total of 100 Sprague Dawley rats were randomly divided into sham operation,ischemia-reperfusion and salidroside 12,24 and 48 mg/kg groups （n =20 in each group）,and than redivided into 6 h,24 h,72 h and 7 dsubgroups （n =5 in each subgroup）.A rat model of global cerebral ischemia was established using the four-vessel occlusion method.Immediately after modeling,all groups were administered intragastrically for 7 days.The brain water content was quantitated by the wet-dry weight method.The neurological evaluation was performed using a neurological deficit score （NDS）.Results After modeling both the ischemia-reperfusion group and all the salidroside groups had significant neurological deficit,and as time went by,it was improved gradually.Compared to the ischemia-reperfusion group at the corresponding time points,neurological deficit in all the salidroside groups was improved significantly （all P 〈 0.05）,and showing a dose-dependent trend.Compared to the salidroside 12 mg/kg and 24 mg/kg groups,neurological deficit in the salidroside 48 mg/kg group was improved significantly at 72 hours and 7 days （all P 〈 0.05）.The brain water contents began to increase at 6 hours after modeling in the the ischemia-reperfusion group and all the salidroside group.They reached the peak at 72 hours,and significantly higher than that in the sham operation group （all P 〈 0.05）.The brain water contents in all the salidroside group were significantly lower than those in the ischemiareperfusion group at 24 and 72 hours after modeling （all P 〈 0.05） and showing a dosedependent trend.The brain water content in the salidroside 48 mg/kg group was close to that in the sham operation group at 7 days after modeling.Conclusions Salidroside may significantly decrease brain edema and improve neurological function in global cerebral ischemia-reperfusion injury in rats,and it has a neuroprotective effect.
出处
《国际脑血管病杂志》
北大核心
2011年第12期896-900,共5页
International Journal of Cerebrovascular Diseases
关键词
红景天苷
脑缺血
疾病模型
动物
脑水肿
神经保护药
大鼠
Rhodioloside
Brain ischemia
Disease models, animal
Brain edema
Neuroprotective agents
Rats
作者简介
通信作者：张犁，Email：zhangli．dr@163．com
