摘要
目的探讨利培酮对神经的保护作用。方法以100 mg/L神经生长因子诱导大鼠PC12细胞7 d后,将细胞随机分为无血清组、氟哌啶醇组、利培酮组(氟哌啶醇组和利培酮组的浓度均分为10,20,40,60,80μmol/L)和血清组,每组5孔。采用四甲基偶氮唑盐(MTT)法测定细胞活性,流式细胞仪检测细胞凋亡率、细胞周期,用Hoechst33342染色法观察细胞形态学变化。结果(1)给予利培酮(20,40μmol/L)72 h后,细胞活性[(64.2±4.4)%,(60.8±3.9)%]高于无血清组[(48.0±2.8)%;P<0.01],而10μmol/L、20μmol/L、40μmol/L、60μmol/L、80μmol/L各浓度氟哌啶醇组的细胞活性[分别为(31.8±3.9)、和(24.4±1.3)%、(14.3±2.6)%、(10.5±2.1)%和(4.1±1.4)%]均低于无血清组(P<0.01)。(2)流式细胞仪检测,利培酮组的凋亡率[(34.6±2.8)%]低于无血清组[(50.7±3.1)%;LSDt-=-16.0,P<0.01],而氟哌啶醇组的凋亡率[(59.3±5.2)%]高于无血清组(LSD-t=8.6,P<0.01);血清组和利培酮组细胞滞留于G1期的比例[分别为(53.5±5.4)%和(71.1±3.7)%]低于无血清组[(81.2±3.0)%]和氟哌啶醇组[(82.1±5.7)%;P<0.01]。(3)无血清组和氟哌啶醇组多见凋亡细胞,其中氟哌啶醇组更明显;而利培酮组偶见凋亡细胞,以核浓缩为主。结论利培酮对PC12细胞有抗凋亡作用,可能是其参与神经保护作用的机制之一。
Objective To investigate the protective effect of risperidone on neuronal cells. Methods PC12 cells differentiation were performed by adding nerve growth factor at the concentration of 100 mg/L for 7 days in vitro, then the cells were randomly divided into four groups： serum-cultured, serumfree, haloperidol and risperidone treatment group. MTT assay was performed to determine cell viability. The apoptotic cell ratio and cell cycle were detected with flow cytometer assay. And the apoptotic morphological changes of PC12 cells were examined with Hoechst33342 staining. The effect of risperidone or halopefidol treatment was compared with that in serum-free group without treatment, respectively. Results （ 1 ） Cell viability in risperidone group at the concentration of 20 [ （ 64.2 ± 4.4） %] or 40 μmoVL [ （ 60.8 ± 3.9） % ] was significantly higher than that in serum-free group [ （48.0 ± 2.8 ） % ] after 72 h in vitro （ P 〈 0.01）. However, Cell viability in haloperidol group at the concentration of 10 [ （31.8 ± 3.9）% 1, 20 [（24.4±1.3）%],40 [（14.3±2.6）%], 60 [（10.5 ±2.1）%], or 80 μmol/L [（4.1 ±1.4）%], was significantly lower than that in serum-free group （P 〈0.01 ）. （2） Apoptotic ratios in risperidone group （34.6±2. 8） % was significantly lower than that in serum-free group [ （50.7 ± 3.1 ） % ; LSD-t = - 16.0, P 〈0.01 ] with flow cytometer assay, however, apoptotic ratios in haloperidol group （59.3 ± 5.2）% was significantly higher than that in serum-free group [ （50.7±3.1 ） % ; LSD-t = 8.6, P 〈 0.01 ]. The proportionof cells detained at G1 stage of cell circle in serum-cultured and risperidone groups [ （53.5 ± 3.4） % anct （71.1 ± 3.7）%, respectively], were lower than that in serum-free and haloperidol groups [ （81.2 ± 3.0） % and （ 82.1 ± 5.7 ） %, respectively ; P 〈 0.01 ]. （ 3 ） More apoptotic cells were observed in serumfree and haloperidol treatment groups, more in the latter, however, few in risperidone treatment group, most of which were characterized by karyopyknosis. Condusion Risperidone might have neuroprotective effect on neurons through rescuing cells from apoptosis.
出处
《中华精神科杂志》
CAS
CSCD
北大核心
2006年第2期106-109,共4页
Chinese Journal of Psychiatry
基金
杨森科学基金资助项目(JRC2004)
作者简介
研究生
通信作者：谭庆荣，Email：tanqingr@fmmu．edu．cn
