摘要
目的观察Livin基因在急性白血病患儿白血病细胞中的表达及其意义。方法采用半定量反转录聚合酶链反应(RT-PCR)方法检测31例ALL及22例AML患儿白血病细胞Livin mRNA的表达水平,并以12例非白血病患儿为对照组。结果急性白血病(AL)患儿白血病细胞Livin mRNA阳性表达率为60.3%。ALL组和AML组Livin mRNA阳性表达率分别为61.3%和59.1%,两者比较差异无统计学意义,但均高于非白血病对照组(8.3%),并且Livin mRNA阳性患儿的缓解率明显低于阴性患儿的缓解率。结论 Livin基因的过度表达与AL的发病具有相关性,可作为AL的诊断、复发及预后判断的指标之一。
Objective To explore the expression of Livin gene in childhood acute leukemic（CAL） cells and its significance. Methods Semi quantitative reverse transcriptase polymerase chain reaction （RT-PCR） was used to detect the mRNA of I.ivin of CAL, including acute lymphoblastic leukemia（ALL, n = 31 ） and acute myeloid leukemia （ALL, n = 22）, and non-leukemia group（n = 12）. Results The positive expression rate of Livin mRNA in CAL was 60.3~ ,and that of Livin mRNA in ALL ceils and AML ceils were 61.3~ and 59.1 ~ ,respectivel. The difference between them was not significant, but higher than that in control group （8.3 ~ ）. And the remission rate of Livin mRNA positive AL children was lower than that of Livin mRNA negative children. Conclusion The overexpression of Livin mRNA plays an important effect on childhood acute leukemic,which can be used as one of the parameters in the diagnosis, prediction of recurrence and prognosis of the disease.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2012年第1期41-43,共3页
Cancer Research on Prevention and Treatment
基金
山东省医药卫生科技发展计划资助项目(2009HW005)
作者简介
李建厂（1972-），男，硕士，副主任医师，主要从事小儿血液及肿瘤性疾病研究
