摘要
目的探讨环氧合酶-2(COX-2)和survivin蛋白在儿童急性白血病(AL)中的表达及意义。方法应用免疫组织化学链霉亲和素-生物素-过氧化物酶(SABC)法检测2006年6～11月收治42例AL患儿骨髓细胞中COX-2和survivin的表达,对照组为10例非恶性血液病且骨髓正常儿童。结果AL患儿COX-2和survivin表达阳性率分别为52.4%和45.2%,与对照组比较差异有显著性(Pa<0.05)。其中32例急性淋巴细胞白血病(ALL)COX-2和survivin的表达阳性率分别为46.9%和40.6%,与对照组比较差异有显著性(Pa<0.05)。ALL高危组和中低危组COX-2阳性表达率分别为54.5%和42.8%,二组比较差异无统计学意义(Pa>0.05)。AL42例COX-2与survivin表达均为阳性16例,均为阴性17例,COX-2和survivin蛋白表达呈正相关(r=0.579P<0.001)。COX-2阴性组初次完全缓解率高于阳性组(93.3%和66.7%),差异有显著性(P<0.05)。结论1.COX-2蛋白在儿童AL中表达增高;2.COX-2与抗凋亡因子survivin共同参与AL发展,二者可能起协同作用;3.COX-2高表达可能与儿童AL预后有关。
Objective To study the expression of cyclooxygenase -2（ COX -2） and survivin in children with acute leukemia （AL） and its significance. Method The expression of COX - 2 and survivin were determined by immunohistochemical SABC assay, Results The ex- pression rate of COX - 2 and survivin were 52, 4 % （ 22/42 cases） and 45.2 % （ 19/42 cases） in A L, and the expression rate of COX - 2 and survivin were 46, 9% （ 15/32 cases） and 40, 6% （ 13/32 cases） and in acute lymphonate leukemia（ALL） , both of them were higher than those in control group（ Pa 〈 0, 05 ）, COX -2 expression rate in high risk ALL and middle risk - low risk ALL were 54, 5% and 42, 8%, respectively, and the difference had no stastical significance（ Pa 〉 0, 05 ）, The positive rate of COX -2 was 84% （ 16/19 cases）in 19 cases with survivin positive expression, and the negative rate of COX - 2 was 85 % （ 17/20 cases） in 20 cases with survivin negative expression, and there was positive correlation between COX - 2 expression and survivin expression （ r = 0.579 P 〈 0.001 ）, The complete remission（CR） rate was higher in COX -2 protein negative patients than that in positive patients （93, 3% vs 66, 7 % ） , and the difference was statistically significant（ P 〈 0.05 ）, Conclusions 1, Expression of COX - 2 and survivin increase in children with AL, 2, COX - 2 and survivin may involve in the development of AL, 3, The overexpression of COX - 2 may have relationship to the prognosis of AL.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2007年第15期1135-1137,共3页
Journal of Applied Clinical Pediatrics
基金
国家自然科学基金项目资助(39970778)
作者简介
宋艳清，女，主治医师，硕士生。现在武汉亚洲心院脏病医院。
通讯作者：胡群，女，教授，博士生导师，研究方向为血液疾病。
