摘要
采用石蜡切片方法,对叶用芥菜细胞质雄性不育系0912A及其保持系0912B的花药发育过程进行细胞学观察,以确定其花药败育的时期、方式和特点。结果表明:0912A不育系的败育形式多样,主要有花药发育受阻于孢原细胞分化期,无花粉囊的形成;部分花药在花粉母细胞期至单核小孢子期出现发育异常:有的花粉母细胞液泡化后浓缩解体,还有的花粉母细胞因绒毡层细胞径向肥大或液泡化而受挤压解体;有的花粉母细胞虽能进行减数分裂,但发育到四分体时期或单核时期细胞解体,花粉败育。同时对0912A的花器官形态进行观察,发现其雄蕊退化为细丝状,败育彻底。
To investigate the spatial-temporal patterns and other characteristics of anther abortion in 0912A as a leaf mustard cytoplasmic male sterile line and its maintainer line 0912B, cytological observa- tions of anther development were made by means of paraffin sections. The results showed that the abor- tion of male sterile line was diverse. The abortion mainly occurred at the differentiation stage of arches- porial cell,which lacked the formation of pollen sacs. Part of anther abortion occurred at the period from pollen mother cell to the mono-nuclear microspore.some pollen mother cells gathered and disintegrated gradually after vacuolization; some pollen mother cells disintegrated due to the hypertrophy of tapetal layer cells in the radial direction; some pollen mother cells could develop to tetrad, then disintegrated~ part of the pollen mother cells could develop to the mono-nucleus microspores,however the mono-nucle- us microspores broke when they were highly vacuolated. The stamens of 0912A were also found to de- generate as abortive filaments.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2012年第1期44-49,共6页
Journal of Huazhong Agricultural University
基金
中央高校基本科研业务费专项资金(2009QC027,2011QC082)
湖北省重点自然科学基金项目(2010CBB01703)
关键词
叶用芥菜
雄性不育
石蜡切片
细胞学
花器官
leaf mustard
male sterile
paraffin section
cytology
floral organ
作者简介
邹瑞昌，硕士研究生．研究方向：蔬菜遗传育种．E-mail：zouruichang@163．com.
通讯作者：万正杰，博士，副教授．研究方向：蔬菜遗传育种．E-mail：wanzj@mail．hzau．edu．cn
