摘要
拟利用非对称原生质体融合技术向芸薹属花椰菜中转移野生抗逆性状。供体Brassica spinescens具有光合效率高,抗白锈病、蚜虫、黑斑病,耐盐等优良特性。用经UV处理的供体叶肉原生质体与花椰菜下胚轴原生质体通过聚乙二醇(PEG)融合,培养后获得379株再生植株。对其中的120株进行过氧化物同工酶分析和RAPD分子标记检测,证明有23株为杂种植株。流式细胞仪倍性分析表明,杂种植株核DNA含量全部高于供、受体亲本核DNA含量总和,植株倍性在四至八倍之间。还对供体不同UV射线处理的剂量进行了初步探索,结果表明0.0750 J.cm-2为最大辐射剂量。
Asymmetric protoplast fusion between was taken for transferring of wild resistant traits into B. Brassica oleracea L. var. botrytis and B. spinescens oleracea. B. spinescens possesses desirable characteristics such as high photosynthetic efficiency, resistance to the white rust （caused by Albugo candida） , aphid and alternaria blights （caused by Alternaria brassicae ） and tolerance to salt. PEG （polyethyleneglycol） induced asymmetric fusions were performed between mesophyll protoplasts of B. spinescens irradiated with UV and hypocotyl protoplasts of B. oleracea L. var. dase isozyme （POX） analysis was taken in 120 botrytis, and 379 regenerated plants were obtained. Peroxiplants, and 23 of them showed hybrid characters that were confirmed by the RAPD marker detection. The relative nuclear DNA content of the hybrids measured by flow cytometry （FCM） showed that all of the 23 analyzed plants had a DNA content from tetraploidy to octoploidy. Influence of UV doses had been tested, and results showed that the maximum UV dose for B. spinescens was 0. 0750 J · cm^-2.
出处
《园艺学报》
CAS
CSCD
北大核心
2005年第6期1039-1044,共6页
Acta Horticulturae Sinica
基金
北京市自然科学基金重大项目(5050001)
关键词
芸薹属
种间杂种
非对称体细胞融合
原生质体培养
杂种鉴定
Brassica
Interspecific hybrid
Asymmetric somatic fusion
Protoplast culture
Hybrid identification
作者简介
刘凡 通讯作者Author for correspondence（E-mail：liufan@nercv．com）
