摘要
以壳聚糖为载体,戊二醛为交联剂,氨基三乙酸为配基,制备固载铜离子的金属螯合亲和吸附剂,层析分离纯化乳酸脱氢酶(LDH),收得率为23.3%,纯化倍数达3.1。实验结果表明该亲和层析固定相对LDH有较高的分离纯化选择性。
With using chitosan as the support,glutaraldehyde as the crosslinking agent and nitrilotriacetic acid as the chelating ligand,affinity adsorbent was prepared by immobilized copper ion.Lactate dehydrogenase（LDH） was purified by chromatography with a recovery rate of 23.3% and purfication fold of the 3.1.The results show that the affinity chromatography has a high selectivity for the separation and purification of LDH.
出处
《分析科学学报》
CAS
CSCD
北大核心
2011年第6期767-769,共3页
Journal of Analytical Science
基金
中南民族大学自然科学基金(XTZ09005)
关键词
亲和层析
乳酸脱氢酶
壳聚糖
Affinity chromatogramphy
Lactate dehydrogenase
Chitosan
作者简介
通讯作者：柳畅先，女，教授，研究方向为富集与分离．
