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新型固定金属离子亲和吸附剂的制备及对蛋白质的吸附研究 被引量:5

Preparation of Novel Immobilized Metal Ion Affinity Adsorbent For Protein Adsorption
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摘要 以β-环糊精(β-CD)键合硅胶为载体,亚氨基二乙酸(IDA)为螯合基,制备了新型固定金属离子亲和吸附剂.通过13C固体核磁、元素分析对其进行了表征.研究了吸附剂对牛血清蛋白(BSA)的吸附特性,25℃时吸附剂对BSA的最大吸附量达35 mg/g吸附剂. A new immobilized metal ion affinity(IMA) adsorbent was prepared by β-cyclodextrin(β-CD) bonded silica gel as the support and iminodiacetic acid(IDA) as the ligand.The prepared adsorbent was characterized by solid state 13C NMR and elemental analysis.The adsorption capability of the adsorbent towards bovine serum albumin(BSA) was then investigated.The maximum adsorption capacity for BSA could reach 35 mg/g adsorbent at 25 ℃.
出处 《河南大学学报(自然科学版)》 CAS 北大核心 2007年第2期152-156,共5页 Journal of Henan University:Natural Science
基金 河南省科技攻关项目(0424270066)
关键词 蛋白质 亲和吸附剂 Β-环糊精 硅胶 protein affinity adsorbent β-cyclodextrin silica gel
作者简介 叶青(1979-),男,河南淅川人,理学硕士,主要从事高分子化学方面的研究. 通讯联系人,E-mail:hdyewenyu@yahoo.com.
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