摘要
南荻是一种高木质纤维素含量的禾本科多年生草本植物,但其中的高木质素含量严重制约了它作为能源植物的开发利用。采用RT-PCR及RACE方法从南荻中分离到G木质素单体形成的关键酶CCoAOMT家族的一个基因,将该基因序列递交到GenBank,登录号为JF965572。该基因全长工1206bp,含有一个786bp的阅读框,编码一个261aa的蛋白,分子量约为29kD。通过NCBI在线比对发现该基因的预测蛋白与同为禾本科玉米的CCoAOMT酶蛋白有很高的同源性,最高达到94.0%;氨基酸序列多重比较的结果表明,该基因编码肽链含有CCoAOMT类基因全部的保守序列元件;系统进化树分析表明,该基因与玉米的CCoAOMT亲缘关系最近。
Triarrhena lutarioriparia L.Liou var.lutarioriparia has high lignocellulosic which can be convert into biofuel.The high content lignin seriously hampered its utilization as an energy plant.It is difficult to degrade lignin.Caffeoyl CoA-O-methyltransferase is a key enzyme for Triarrhena lutarioriparia L.Liou var.lutarioriparia to formate the G monomer.The caffeoyl CoA-O-methyl-transferase gene from Triarrhena lutarioriparia L.Liou var.lutarioriparia was cloned by RT-PCR and RACE（GenBank accession number： JF965572） and was analyzed from an angle of bioinformatics.The gene contains a reading frame（ORF） which encodes a polypeptide of 261 amino acids with predicted molecular mass of 29 kD.The results of sequence analysis show that the deduced polypeptide contains all the conserved sequences of CCoAOMT family and it was highly homologous to CCoAOMT proteins from Zea mays with 94.0% identity.Phylogenetic tree analysis also indicte that the gene was more related to the CCoAOMT of Zea mays.
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2011年第10期94-99,共6页
Journal of Central South University of Forestry & Technology
基金
973前期项目资助项目(2010CB134403)
湖南农业大学校青年基金项目(07QN30)
作者简介
洪艳云（1973-），女，湖南邵东人，讲师，博士研究生，研究方向：植物生理与分子生物学；电话：15116346538；E-mail：hongyanyun@126．com
通讯作者：易图永（1971-），男，湖南洪江人，教授，博士生导师；E-mail：yituyong@hotmail.com
