摘要
以埃坡霉素产生菌粘细菌纤维堆囊菌为出发菌株,经亚硝基胍诱变处理后,在含有前体丙酸盐的平板中筛选抗性突变株,得到一株高产突变株N-19,埃坡霉素产量为18.5 mg/L,比出发菌株提高了69.7%。通过优化发酵培养基的碳、氮源组成,埃坡霉素产量达36.1 mg/L,此时埃坡霉素B和埃坡霉素A的比值为0.4;在摇瓶发酵过程中补加前体丙酸盐,埃坡霉素B和埃坡霉素A的比值增至2.1。
The epothilones-producing strain Sorangium cellulosum was mutated with NTG and screened in the plate containing propionate,the precusor of epothilones.A mutant N-19 with the propionate resistant was obtained,whose production of epothilones was 18.5 mg/L,an increase of 69.7% compared with that of the parent strain.Through further optimization of the fermentation medium,the yield of epothilones was 36.1 mg/L and the ratio of epothilone B to epothilone A was 0.4.After bacth-feeding of propionate during the fermentation process in shaking flask,the ratio of epothilone B to epothilone A increased to 2.1.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2011年第4期258-261,共4页
Chinese Journal of Pharmaceuticals
基金
国家"重大新药创制"创新药物研究与开发技术体系建设(2008ZX09401)
国家"重大新药创制"科技重大专项资助(2009ZX09301-007)
作者简介
王向阳（1985-），男，硕士研究生，专业方向：微生物与生化药学。通信联系人：陈少欣（1972-），男，研究员，从事微生物与生化药学研究。1bl：021-62479808x712 E—mail：sxzlb@263．net
