摘要
目的探讨奥沙利铂(LOHP)联合低分子柑橘果胶(LCP)对结肠癌HCT116细胞增殖的抑制作用及其机制。方法 MTT法检测LOHP单药及LOHP联合LCP对HCT116细胞增殖的影响;流式细胞仪检测以上两组给药处理HCT116细胞的凋亡比例以及细胞周期的变化;应用Western blot分析procaspase-3,-8,-9,PARP的变化。结果两实验组对HCT116细胞增殖均有抑制作用,联合组抑制作用较LOHP组更为显著(P<0.05)。两组均能使HCT116细胞凋亡比例增加;联合组较LOHP组细胞凋亡更显著(P<0.01)。两组均出现G0/G1期细胞减少,G2/M期细胞增加;联合组较LOHP组增加更为显著(P<0.01)。两组均出现procaspase-3,-9,PARP蛋白的表达下调,联合组较LOHP组下调更加明显;两组间procaspase-8表达无明显差异。结论 LCP能增加LOHP对HCT116细胞增殖的抑制作用,该效应可能与细胞周期的调节以及活化线粒体凋亡途径有关。
Objective To investigate the inhibitory effect of the combined treatment with oxaliplatin（LOHP） and low molecular weight citrus pectin（LCP） on the proliferation of human colon cancer cell line HCT116 and its mechanism. Methods The effect of LOHP alone and LOHP combined with LCP on the proliferation of HCT116 cells was determined by MTT assay.The apoptosis rate and cell cycle of HCT116 cells after the above two treatments were detected by Flow cytometry（FACS）,and the changes of procaspase-3,-8,-9 and poly（ADP-ribose） polymerase（PARP） of the cells after treatments were analyzed by Western blot. Results Both treatments had inhibitory effect on the proliferation of HCT116 cells,and the combination of LOHP and LCP exerted stronger inhibitory effect than LOHP alone treatment（P0.05）.FACS analysis showed that both treatments increased the apoptosis rate of HCT116 cells,and the apoptosis rate of the combined treatment was significantly higher than that of the LOHP alone treatment（P0.01）.Both treatments decreased the G0/G1 phase and increased G2/M phase of the cells,while the G2/M phase ratio of the combined treatment was increased more significantly higher than that of the LOHP alone treatment（P0.05）.The expressions of procaspase-3,-9,and PARP protein were all down-regulated after both treatments,and those down-regulations after the combined treatment were more obvious compared with those after LOHP alone treatment.The expression of procaspase-8 showed no obvious difference after the two treatments. Conclusions LCP can increase the inhibitory effect of LOHP on proliferation of human colonic cancer cell line HCT116,and this effect may be related to modulation of the cell cycle and activation of mitochondria aboptosis pathway.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2011年第10期1053-1057,共5页
China Journal of General Surgery
基金
广东省科技计划项目(2010B030700045)
关键词
结肠肿瘤/病理学
奥沙利铂
低分子柑橘果胶
细胞增殖
Colonic Neoplasms/pathol
Oxaliplatin
Low Molecular Weight Citrus Pectin
Cell Proliferation
作者简介
汪峰，九江学院附属医院住院医师，从事消化道肿瘤基础与临床方面研究
通讯作者：刘海鹰 E-mail：gzdlhy@yahoo．com
