摘要
为了研究引入秸秆碳源对根结线虫(Meloidogyne spp.)病害严重土壤中微生物生物量和原生动物的影响,以番茄为供试作物,设置4个梯度的小麦秸秆添加量[CK(0 g.kg-1),1N(2.08 g.kg-1)、2N(4.16 g.kg-1)和4N(8.32 g.kg-1)],研究不同种植时间(6个月和4个月)下土壤微生物生物量碳、氮和原生动物丰度的变化。研究结果表明:添加秸秆对微生物生物量碳、氮和原生动物丰富度有明显促进作用,添加的秸秆量越多,这种促进作用越明显。不同秸秆添加量处理中,微生物生物量碳、氮和原生动物丰度为:4N>2N>1N>CK。秸秆对原生动物的群落结构也有显著影响,在各处理中,鞭毛虫和肉足虫占有绝大比例,分别占总丰度的29.44%和66.19%,纤毛虫仅占4.37%。在相同添加秸秆量条件下,土壤原生动物丰度随种植时间的延长而提高,而微生物生物量碳、氮量随种植时间的延长而降低。而在种植时间相同条件下,随着秸秆量的增加土壤微生物生物量碳、氮量和微生物生物量碳氮比和原生动物总丰度相应增加。
Driven by economic profits, the area of vegetables grown under plastic-film greenhouse has rapidly increased in recent years in China. Specific environmental conditions in plastic-film greenhouses could cause severe root-knot nematode disease. Hence a greenhouse pot experiment was conducted to study the effects of added straw carbon on soil microbial biomass carbon （MBC）, microbial biomass nitrogen （MBN） and soil protozoa abundance. In the experiment, independent cultivation methods and selected tomato crops were used in a 3-gradient additive [IN （2.08 g.kg-1）, 2N （4.16 g.kg-l） and 4N （8.32 g.kg-1）] of winter wheat straw. Two sets of experiments were set up with respective tomato growth durations of 6 and 4 months, Based on the study, the order of abun- dance of MBC, MBN and protozoa was 4N〉2N〉IN〉CK. Wheat straw carbon significantly enhanced MBC, MBN and protozoa abundance and protozoa community structure. Flagellate and amoeba were in vast proportions of soil microbial abundance, respectively accounting for 29.44% and 66.19%. Ciliates accounted for only 4.37% of total soil microbial abundance. With increasing cul- tivation period, soil protozoa abundance increased while soil MBC and MBN decreased under the same additive amount of wheat straw carbon. Under the same cultivation period, MBC, MBN, MBC/MBN ratio and soil protozoa abundance increased with increasing amount of added wheat straw carbon.
出处
《中国生态农业学报》
CAS
CSCD
北大核心
2011年第6期1283-1288,共6页
Chinese Journal of Eco-Agriculture
基金
国家自然科学基金项目(30970536)资助
关键词
秸秆碳源
微生物生物量碳
微生物生物量氮
原生动物丰富度
温室蔬菜
Straw carbon source, Microbial biomass carbon, Microbial biomass nitrogen, Protozoa abundance, Greenhouse vegetable
作者简介
张四海（1981-），男，博士研究生，主要研究方向为土壤生物多样性和土壤食物网。E-mail：zhangsihai2352@163．com
通讯作者：曹志平（1962-），女，博士，教授，主要研究方向为土壤生态学。E-mail：zhipingc@cau．edu．cn
