摘要
目的:建立测定复方苦参注射液中聚山梨酯80含量的气相色谱方法。方法:采用脂肪酸甲酯化法进行样品前处理。气相色谱条件:DB-WAX毛细管柱(PEG-20000,30 m×0.32 m×0.25 m);氢火焰离子化检测器(FID);进样口温度250℃,检测器温度为250℃;柱温初始温度为80℃,以10℃.min-1升温至220℃,保持20 min;载气为氮气,恒定流速1 mL.min-1,进样方式为分流进样,分流比10∶1;进样量为1μL。结果:聚山梨酯80的进样浓度在0.1～5.0 mg.mL-1(r=0.9998)范围内呈良好线性关系;平均回收率(n=3)为97.4%～100.7%。结论:该方法简便快速,可用于复方苦参注射液中聚山梨酯80的含量测定。
Objective：To establish a GC method for determination of polysorbate 80 in compound kushen injection.Methods：The gas chromatographic analysis was performed using Capillary column DB-WAX（PEG-20000,30 m×0.32 m×0.25 m）.The detector was FID.Inlet temperature was 250 ℃ and the detector temperature was 250 ℃.Program temperature was used with initial column temperature of 80 ℃,and increasing to 220 ℃ at the rate of 10 ℃·min^-1,holding for 20 min.highly pure N2 was adopted as carrier gas at the flow rate of 1 mL·min^-1.Split injection with split ratio of 10∶1.Injection volume was 1 μL.Results：The linear range for polysorbate 80 was 0.1-5.0 mg·mL-1（r=0.9998）;The average recoveries（n=3） were 97.37%-100.70%.Conclusion：This method is simple,quick and specific.It can be applied for determination of polysorbate 80 in compound kushen injection.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2011年第10期1993-1995,共3页
Chinese Journal of Pharmaceutical Analysis
关键词
复方苦参
中药注射液
药用辅料
聚山梨酯80
气相色谱法
Chinese materia medica injection
compound Kushen
pharmaceutic adjuvant
polysorbate 80
GC method
作者简介
第一作者Tel：13935522309；E—mail：1yy7273@163．com
