摘要
以预榨浸提棉籽粕为原料,先选用戊聚糖复合酶、糖化酶等植物水解酶对棉籽粕进行作用,再进一步用乙醇溶液浸洗制备棉籽浓缩蛋白。结果表明:在添加糖化酶1 600 U/g、戊聚糖复合酶0.60 fbg/g、料液比1∶10和温度50℃条件下酶作用140 min后,再以料液比1∶7、体积分数70%乙醇溶液和温度60℃条件下醇洗2次,每次45 min,得到产品浓缩蛋白的蛋白质质量分数为64%,蛋白质收率为78%。
The pre-pressed cottonseed meal as material was firstly hydrolyzed by pentosan compound enzyme and glucoamylase, and then washed by ethanol to preparate protein concentrate. The result illustrated as that： Under the condition of adding glueoamylase 1 600 U/g, pentosan enzyme 0.60 fbg/g, solvent/solid ratio of 1 ： 10, temperature 50~C, the cottonseed meal was hydrolyzed for 140 rain, and then washed by 70% （v/v） ethanol at 60~C dipped for 2 times（once for 45 rain）, with solvent/solid ratio of 1 ： 7. The pro tein content of cottonseed protein concentrate prepared was 64~, and the yield of protein was 78~.
出处
《粮食与饲料工业》
CAS
北大核心
2011年第9期50-52,共3页
Cereal & Feed Industry
关键词
棉籽粕
棉籽浓缩蛋白
酶解
醇洗
戊聚糖复合酶
糖化酶
制备
cottonseed meal
protein concentrate
alcohol wash
pentosan compound enzyme
glucoamylase ~ preparation
作者简介
潘晶（1985一），女，硕士研究生，主要从事植物蛋白方面的研究工作。
通讯作者：张晖（1966），女，教授，研究方向为谷物功能成分与健康食品。
