摘要
目的研究甘肃地区男性不育患者Y染色体AZF区域微缺失的频率、分布情况方法采用多重PCR技术,对甘肃地区373例男性不育症患者进行Y染色体AZFa,AZFb,AZFc三个区域6个STS位点的微缺失检测。结果 373例男性不育患者中,42例发生了STS位点缺失,缺失率11.3%。其中无精子症因子AZFa(SY86,SY84)区未见缺失;AZFb(SY127,SY134)区缺失4例(9.52%);AZFc(SY254,SY255)区缺失32例(76.2%);AZFb+c区缺失5例(11.9%);AZFa+b+c缺失1例(2.38%)。结论甘肃部分地区AZF区域微缺失的频率、分布与国内其他报道一致,但是在本研究中未检测到AZFa区缺失患者。
Objective： To investigate the frequency and distribution of Y chromosome microdeletion in male patients with infertility in Gansu province.Methods： Multiplex PCR was preformed within the Azoospermia factor（AZF） region to detect Y chromosome microdeletion in 373 infertile male patients.Results： In 373 infertile male patients,42（11.3%） were found to have Y chromosome microdeletion in AZF regions.Among the 42 cases,none deletion was in AZFa,4（5.93%） deletions were in AZFb,32（76.2%） deletions were in AZFc and 5（11.9%） deletions were in AZFb＋AZFc,1（2.38%） deletions were in AZFa＋AZFb＋AZFc.Conclusion： The frequency and distribution of Y chromosome microdeletion and male azoospermia in GanSu Province is almostly consistent with the other research.
出处
《中国优生与遗传杂志》
2011年第10期43-45,91,共4页
Chinese Journal of Birth Health & Heredity
