摘要
制备了2-氨基嘧啶缩邻香草醛希夫碱配体C12H11N3O2(HL),将其与Cu(Ⅱ)作用合成了1种新型的铜配合物{[CuL(NO3)(C2H5OH)].2H2O}。通过元素分析、红外光谱、紫外-可见光谱、TG-DTG及摩尔电导分析等手段对合成的配合物进行了表征,推测了配合物可能的结构,铜为六配位,八面体结构:配体中-C=N-上的N原子、嘧啶环上的N原子、酚羟基上的O原子均参与了配位,配合物中硝酸根以双齿形式参与配位,溶剂分子以配位形式存在,水以结晶形式存在。利用紫外-可见吸收光谱、荧光光谱、黏度测试、电化学方法研究了配合物与DNA的相互作用,实验结果表明,配合物与DNA之间通过插入作用结合,并得出配合物与DNA的结合常数为Kb=8.12×104L.mol-1。
This paper reports the synthesis of Cu（Ⅱ） complex {[CuL（NO3）（C2H5 OH）]·2H2O} with ligand derived from the condensation of o-vanillin with 2-amino pyrimidine in the absolute alcohol. It is characterized by elemental analysis,IR spectrometry, UV-Vis spectrometry ,TG-DTG and molar conductance. The suggested structure of the complex is concluded. The copper ion is six-coordinated with a octagonal geometry. The two nitrogen atom（one from the -C=N- , the other from the pyrimidine） ,oxygen atom of hydroxybenzene in ligand is coordinated to the copper ion. The nitric acid is coordinated to the copper ion in bidentate fashion, and the oxygen atom of C2 Ha OH is coordinated to the copper ions, too. Water molecules are existing as crystal water. The interaction of the Cu（ Ⅱ ） complex with DNA has been investigated by UV-Vis spectrometry, fluorescence spectrometry, viscosity and electrochemical behavior. The rusults show that the interaction of the Cu（ Ⅱ ） complex with DNA belong to intercalation mode ,and the binding constant for the complex is Kb=-8. 12×10^4L · mol^-1.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第9期77-82,共6页
Periodical of Ocean University of China
基金
国家自然科学基金项目(2097111521071134)资助
作者简介
郑玉萍（1984-），女，硕士生。Email：yuping8288@163．com
通讯作者：E-mail：fanyh@one．edu．cn
