摘要
应用紫外、荧光、黏度等方法, 对二(2-苯并咪唑亚甲基)胺合铜(II)配合物与小牛胸腺 DNA 作用方式进行了研究.配合物与 DNA 作用时, 使紫外吸收明显减色, 荧光降低, 黏度降低; Scatchard 图表明配合物对溴化乙锭(EB)与 DNA的结合为非竞争性抑制. 实验结果表明, 配合物与 DNA 作用方式可能为静电结合.
The interaction of the LCu(ClO4)2 [L=bis(2-benzimidazolylmethyl)amine] with calf thymus DNA has been investigated using UV spectra, fluorescent spectra, and viscosity. Hypochromism was ob- served in the UV spectra of DNA in the presence of the complex, and decreased fluorescence was observed for complex with native and denatured DNA addition. The relative viscosity of DNA decreased with addition of the complex. The Scatchard plots show uncompetitive inhibition of EB binding to DNA. The experimen- tal results show that the mode of binding of the complex to DNA is electrostatical binding.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2005年第1期71-74,F008,共5页
Acta Chimica Sinica
基金
国家自然科学基金(No. 20171031)
山西省自然科学基金(No. 20011007)资助项目.
