摘要
从石油污染土壤中富集分离、筛选出3株高效降解石油的微生物菌株,通过生理生化特性研究及16SrRNA基因序列分析,确定3株菌均属于红球菌属(Rhodococcus sp),研究和比较了它们与实验室保存的4株菌(分别属于Gordonia sp,Comamonas sp,Pesudomonas sp)降解石油的能力。这7株菌株对石油的不同组分具有不同的降解能力,对7株菌进行不同的组合用以研究复合菌群对石油的降解。结果表明,由两株Rhodococcus sp,一株Gordonia sp和一株Pesudomonas sp组成的复合菌群D,降解石油的能力超过任何单一菌株和其他组合菌群。混合菌群D在5d的培养中能降解70.3%的石油总量和71.4%的芳香化合物。混合菌群D能降解99.8%的C13-19烷烃,92.6%的C20-26烷烃,82.2%的C27-32烷烃以及90.2%的植烷。在实验室模拟条件下,对土壤中石油的降解率达到50%以上。降解土壤中石油的最适温度为10～30℃、pH值为6.5～9.5,接种量需要在106CFU·g-1以上。
Three bacterial strains were isolated from petroleum-contaminated soil and identified as Rhodococcus sp.based on their 16S rRNA gene sequences and morphological,biochemical and physiological characteristics.Abilities to degrade petroleum were studied and compared among three isolates and four strains instored in our laboratory.The seven strains prefered to degrade different component of petroleum respetively.Multiple bacterial consortia were composed by different combination among the seven strains.Consortium D which consists of two strains of Rhodococcus sp,a Gordonia sp and a Pesudomonas sp showed much higher degradating-oil efficiency than any single strains and other consortia.Consortium D could degrade 70.3 % of petroleum totally and 71.4% of aromatic component in petroleum in 5 days incubation.Consortium D could also degrade 99.8% of C13-19 alkanes,92.6 % of C20-26 alkanes,82.2% of C27-32 alkanes and 90.2% phytane in petroleum.When consortium D used in bioremediation of petroleum-contaminated soil,it could remove more than 50% petroleum in soil.In condition of 10~30 ℃,pH 6.5~9.5 and inoculum106 CFU·g-1,the consortium D could degrade petroleum faster.The results indicated that Consortium D had potential used in bioremediation of petroleum-contaminated soil.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2011年第8期1567-1572,共6页
Journal of Agro-Environment Science
基金
国家自然科学基金面上项目(30870081)
关键词
石油污染土壤
降解菌
复合菌群
生物修复
oil polluted soil
degrading bacteria
multiple flora
bioremediation
作者简介
赵硕伟（1960-），男，江苏镇江市人，学士，高级工程师，主要从事农业环境科学研究。
通讯作者：沈标E-mail：shenbiao@njau．edu．cn
