摘要
观察没食子酸丙酯（Propyl gallate，PG）对人慢性髓系白血病K562细胞株的增殖抑制及诱导凋亡作用．采用倒置显微镜观察细胞形态及数目的变化，乳酸脱氢酶（Lactate dehydrogenase，LDH）释放检测细胞活性，流式细胞术PI染色法及DNA倍体分析检测细胞凋亡作用．结果表明，没食子酸丙酯能有效地抑制K562细胞增殖，药物处理24h时细胞形态发生变化，细胞数目减少；细胞培养液中LDH活性分析显示，20-300μg／mL没食子酸丙酯处理24h对细胞毒性作用不明显；但处理时间达48h时，没食子酸丙酯对细胞毒性明显增加；亚二倍体峰（凋亡峰）的出现及DNA片段化分析表明，200μg／mLPG处理细胞24h时，能引起K562细胞凋亡．结果表明，PG具有明显的体外抗肿瘤活性，其抗癌活性与其抑制细胞增殖、诱导细胞凋亡有关．
The study was aimed to investigate the effect of propyl gallate （PG） on proliferation inhibition and apoptosis induction in human chronic myeloid leukemia cell line （K562 cells） in vitro. After exposed to PG, morphological changes was detected by inverted microscope and cell number was counted with a hemocytometer. The lactate dehydrogenase （LDH） activity in cell culture was detected by using ultraviolet spectrophotometer with 340 nm. PG-induced apoptosis of K562 cells was analyzed by DNA fragmentation analysis and flow cytometry labeled by propidium iodide （PI）. PG showed significant growth inhibition of K562 cells in a dose-dependent manner. Typical morphological change was displayed in PG-treated K562 cells for 24 h. There was no obvious change in LDH activity in cell culture when the cells were treated with PG in the range of 20-300 μg/mL. However, the LDH activity increased significantly when the cells were incubated with PG from 24 h to 48 h. DNA fragmentation and sub-G1 apoptotic peak analysis indicated that PG induced apoptosis in K562 cells. Fig 5, Ref 18
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2011年第4期517-520,共4页
Chinese Journal of Applied and Environmental Biology
作者简介
通讯作者Corresponding author（E—mail：dulinfang@yahoo.com）
