摘要
[目的]研究人生长激素(hGH)对胃和结肠癌细胞株DNA倍体的影响。[方法]收集四株胃和结肠癌细胞株 ,以顺铂和/或不同浓度的hGH体外培养 ,24h后以流式细胞仪测定DNA含量 ,根据DNA含量直方图所示G0/G1 期荧光峰均道值计算DNA倍体数。[结果]hGH处理后此几株癌细胞的DNA倍体数未见显著增高 ,加用顺铂后MGN 45胃癌株DNA倍体数显著下调。
To study effect of human growth hormone (hGH) on DNA index of human gastric and colonic cancer cell lines in vitro.Cell lines were cultured with or without DDP for 24 h in hGH at concentration of 50,100 or 200ng/ml,respectively.DNA index was analyzed by flow cytometry.No significant increasing of hGH on DNA index were found in four cell lines.DNA index in human gastric cancer cell line MGN 45 decreased when DDP was added to the culture medium.[Conclusion]The application of hGH in vitro has no significant influence to DNA index in gastric and colonic cancer cell lines.
出处
《肿瘤学杂志》
CAS
2001年第5期286-287,共2页
Journal of Chinese Oncology
关键词
生长激素
胃肿瘤
结肠肿瘤
DNA倍体
实验性
药物治疗
growth hormone
stomach neoplasms
colonic neoplasms
DNA index
neoplasms,experimental
