摘要
目的:建立测定麦冬中沿阶草酮甲的高效液相色谱法。方法:色谱柱:Hypersil C18柱(150mm×4.6mm,5μm);流动相:甲醇-水(75∶25);流速1.0mL/min;检测波长298nm。结果:沿阶草酮甲进样量在0.918～5.508μg范围内与峰面积线性关系良好,回归方程Y=3645282X-420010(r=0.99976),平均回收率为99.80%,RSD=1.74%(n=6)。结论:所建立的高效液相色谱法简便、快速、重复性好,可用于麦冬中沿阶草酮甲的质量控制。
Objective：To establish HPLC method for the content determination of ophiopogonanone A in Radix Ophiopogonis.Methods：The analysis was carried out on Hypersil C18 column （150 mm × 4.6 mm,5 μm）.The mobile phase was MeOH-water（75∶25） with flow-rate of 1.0 mL/min.The eluate was detected at 298 nm in room temperature.Results：The method was simple and had a good linear relationship.The linear range of ophiopogonanone A was 0.918 ～ 5.508 μg （r = 0.999 76）.The recovery was 99.80% （RSD = 1.74%）.Conclusion：The method was simple and suitable for the content determination of ophiopogonanone A in Radix Ophiopogonis.
出处
《中国执业药师》
CAS
2011年第8期28-29,共2页
China Licensed Pharmacist
基金
国家科技重大专项课题(2010ZX09401-306-2-23)
重庆市科委产业化项目(CSTC
2008AB5116)
关键词
高效液相色谱
麦冬
沿阶草酮甲
含量测定
HPLC
Radix Ophiopogonis
Ophiopogonanone A
Content Determination
作者简介
侯浣波，女，主管药师。研究方向：临床药学。
周慧。女，讲师。研究方向：微生物与生化药学。通讯作者E-mail：zhouhui@cqut，edu．cn
