摘要
目的观察富血小板纤维蛋白(PRF)的细胞组成和三维结构并讨PRF凝胶和膜的不同。方法 5名志愿者每人采肘静脉全血两份,随机放入A组(PRF凝胶)和B组(PRF膜),分别进行光镜和扫描电镜(SEM)观察。结果 PRF凝胶中纤维蛋白聚集形成疏松的立体网络结构,有大量的血小板和白细胞分布并呈特定的三维分布,而PRF膜中纤维蛋白立体网络结构坚实致密,以致区分其中的细胞成分有一定的困难。结论 PRF凝胶和膜除了纤维蛋白浓密不同外均聚集了全血中大量的血小板和白细胞,因此,只要遵循主要的生产规则就可以得到一个自体纤维素生物材料。
【Objective】 To observe the cell composition and architecture of the PRF clot and to discuss the structural and morphologic differences between PRF clot and membrane.【Methods】 In the experimental group 5 healthy donors.Whole blood was collected from their elbow vein,that each whole blood in two copies,which were randomLy divided into group A（PRF clots） and group B（PRF membrane）,then the samples were observed by optical microscope and SEM.【Results】 In PRF clot,fibrins get together for a porous three-dimensional network structure,lots of platelets and leucocytes were trapped and showed a specific three-dimensional distribution.In PRF membrane,the fibrin network was very solid and dense,so it is difficult to distinguish from composition of cells.【Conclusion】 Besides the difference of the fibrin dense The PRF concentrated most platelets and leukocytes from a blood harvest.So this protocol offers an autologous ？brin biomaterial as long as the main production principles are respected.
出处
《中国医学工程》
2011年第7期65-67,共3页
China Medical Engineering
关键词
富血小板纤维蛋白
扫描电镜
光镜
platelet-rich fibrin
scanning electron microscope
optical microscope
作者简介
通讯作者：王稚英，Email：bdwzy@126．com
