摘要
目的开发能够快速检测野生尖音库蚊种群对有机磷杀虫剂的抗性水平及靶标抗性的试剂盒。方法根据蚊虫抗药性的生化和分子机制,通过建立不同抗性水平的品系,将经典的羧酸酯酶显色与抗性水平对应起来;根据G119S突变导致的不敏感乙酰胆碱酯酶1对不同浓度残杀威抑制的反应,建立靶标抗性与显色反应的关系。结果制备了反映不同有机磷抗性倍数的羧酸酯酶滤纸显色卡,可以快速、简便的检测野生尖音库蚊种群的有机磷抗性水平;建立了用肉眼就可辨别的乙酰胆碱酯酶显色反应体系,快速检测种群中是否存在靶标抗性及其基因型。结论为我国蚊虫抗性检测和预警工作提供了方便、简洁而有效的工具。
Objective To develop a rapid detection kit for resistance level and target resistance to organophosphate insecticides in Culex pipiens complex field populations. Methods Strains with different resistance levels were established according to biochemical and molecular resistance mechanisms in mosquitoes, to reflect the correlation between typical acetylcholinesterase （ACHE） staining and the resistance staining. The relationship between target resistance and chromogenic reactions was determined according to the inhibitory effects of propoxur at different concentrations on insensitive AChE 1 induced by a Gll9S mutation. Results A set of coloration cards made from AChE filter paper were developed to describe different organophosphate resistance ratios, which could be used for rapid and simple detection of the resistance level in the field. A chromogenic system was established to visualize the presence of target resistance and the relevant genotypes in the population. Conclusion This study introduces a simple and effective tool for detection and monitoring of mosquito resistance.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2011年第4期332-335,共4页
Chinese Journal of Vector Biology and Control
基金
国家科技重大专项课题(2008ZX10004-010)
中国科学院动物研究所知识创新工程青年人才领域前沿项目(KSCX3-IOZ-1006)~~
关键词
有机磷杀虫剂
抗药性
快速检测
羧酸酯酶
乙酰胆碱酯酶
Organophosphate insecticide
Insecticide resistance
Quick detection
Esterase
Acetylcholinesterase
作者简介
崔峰，Email：cuif@ioz．ae．cn；
刘洋洋（1987-），女，在读硕士，从事植物一有害生物一天敌相互关系研究。Email：yangyangguai20@163．com
郎明林，Email：minglinlang@yahoo．eom．cn
崔峰，Email：cuif@ioz．ac．cn；
