摘要
为探明茶叶提取物———茶多酚复合物（ＴＰＰ） 抗肿瘤作用及其机理；采用脱咖啡碱的高纯度ＴＰＰ进行动物体内外抑瘤作用和对细胞免疫功能影响的实验测定。结果表明：ＥＡＣ 或Ｓ１８０ 鼠分别ｉｇ４０ ，８０ 和１２０ｍｇ／（ｋｇ·ｄ） 的ＴＰＰ，对ＥＡＣ的抑制率分别为６５－３ ％ ，５１－０ ％ 和４８－７ ％ ；对Ｓ１８０ 的抑制率为６６－３ ％ ，６８－５ ％ 和５９－８ ％ ；ＥＡＣ 鼠的胸腺指数比对照组分别提高５０－７ ％ ，４０－６ ％ 和１９－８ ％ ；脾指数提高３４－４ ％ ，３１－１ ％ 和２０－０ ％ ；Ｓ１８０ 鼠的胸腺指数也分别提高了６６－２ ％ ，４７－２ ％ 和４８－１ ％ ；脾指数也略有增高。离体细胞培养测定ＴＰＰ对致敏Ｔ淋巴细胞的粘瘤和钻瘤活性，结果显示：各ＴＰＰ组ＥＡＣ鼠的胸腺或脾淋巴细胞对自体瘤细胞的ＡＴＩ和ＥＴＩ均明显高于对照组（Ｐ＜ ０－０１） 。正常小鼠ｉｇ６０ｍｇ／（ｋｇ·ｄ）的ＴＰＰ，连续给药１２ｄ 后，腹腔Ｍ吞噬ＣＲＢＣ的百分率和吞噬指数比对照组分别增加６９－６ ％ 和６８－７ ％ 。因此认为ＴＰＰ不仅具有明显抑制肿瘤的生长，而且还能增强和调节机体的非特异性免疫功能。
The dynamic changes of celluar immune function and anticancer effect of tea polyphenols (TPP) in the mice bearing EAC and S180 tumor were investigated The results showed that at the doses of 40,80 and 120mg/kg·d 1 by ig administration for 10 days in the mice bearing tumor, the TPP can inhibit the EAC and S180 tumor growth, markedly increase their thymus and splenic lymphocytes adhering to tumor cell and emperipolesis index in vitro cultures ( P <0 01) The experimental results also indicated that the TPP could increase the phagocytosis of macrophage, at a dose of 60mg/kg by ig administration for 12 days in normal mice It suggested that anticarcinogenic mechanism of TPP possibly included both cellular immune function and inhibition of tumor growth
出处
《药物生物技术》
CAS
CSCD
1999年第4期212-217,共6页
Pharmaceutical Biotechnology
基金
浙江省高校科研启动基金
关键词
茶多酚
抑癌作用
免疫功能
Tea polyphenols, Antitumor effect, Immunologic function, Emperipolesis
